pE-800fura with Liquid Light Guide Attachment

Overview

Building on the successful pE-800 Illumination System, the pE-800fura offers the most comprehensive calcium imaging system available. It also enhances a range of applications from pH monitoring and optogenetics to everyday fluorescence microscopy, combining lightning-fast switching with eight individually controllable channels. Backed by CoolLED’s world-renowned support and generous warranty, the pE-800fura presents the go-to illuminator for calcium imaging and beyond.

2107002 pE800fura Spectrum v3 01
True 8-channel illumination

Eight powerful and efficient LEDs offer spectral coverage from 340-635 nm, tailored to all popular calcium indicators, pH indicators, opsins, as well as fluorophores for everyday fluorescence ranging from Fura-2 to Cy5.

Individually controlling all eight channels is achieved via USB, TTL or analogue for easily matching each LED to the selected calcium indicator or fluorophore set.

Unlike lasers, the broader spectral output of LEDs can be refined by the use of optical filters in order to excite a range of dyes, expanding the coverage of the pE-800fura. The ability to customise the wavelength output with both wavelength switching and optical filters means experiments are no longer limited by the light source, even as requirements evolve or new dyes emerge in the future.

Offering high-speed wavelength selection across the spectrum, the pE-800fura presents the first direct LED upgrade to traditional monochromator solutions with all the benefits of LED illumination.

We’ve got you covered: Unlike narrow-band illuminators such as lasers, broader LED wavelengths can be optimised with optical filters to excite a range of fluorophores. Suitable LEDs are shown for each fluorophore below, and we can recommend the best optical filters.

 

pE 800fura dyes LED Filter table v4

Accelerating calcium analysis

Industry-leading <7 µs TTL triggering is a breakthrough for ratiometric fluorescence applications such as calcium measurements, maximising the accuracy of measuring ratio pairs. For live cell imaging, capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8-FR (USB controlled TTL trigger box).

TTL triggering graph

Even optical filtering is optimised for speed, with the simplicity of fitting single-band excitation filters at each LED channel avoiding the use of moving parts.

When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for slow and expensive external filter wheels and presents a low-cost approach to high-speed imaging.

pE 800fura Excitation filter holder

Affordable automation with Sequence Runner

Taking speed one step further, Sequence Runner mode combines the versatility of software with the <7 µs speeds of TTL, with minimal requirement for expensive electronic hardware. All you need is a TTL-out. Once an LED and irradiance sequence are specified in software, Sequence Runner provides high-speed synchronised triggering via the global TTL-in of the pE-800fura and a single TTL-out from a camera (or other hardware).

When combined with inline filters, the pE-800fura transforms a manual microscope into an affordable and powerful eight-channel automated system for calcium imaging, ratiometric assays and everyday fluorescence.

Protecting live samples

Photodamage can have a serious impact on live cell data, and the pE-800fura has been carefully designed to overcome this, protecting samples and helping researchers generate the highest quality data.

Industry-leading <7 µs hardware synchronisation protects against photobleaching and phototoxicity by restricting sample exposure to image acquisition. Balancing irradiance to the lowest level possible while still maintaining image quality is another approach for reducing photodamage, and the pE-800fura simplifies this with fine irradiance control from 0-2%, and in 1% steps up to 100%.

Not everyone realises that excitation filters can still leak unwanted light, reducing the signal-to-noise ratio. The ability to select a single LED channel overcomes this, and has the added benefit of allowing a lower concentration of dyes such as Fura-2, which improves cell health and viability due to reduced toxicity – and also reduces reagent cost.

These factors not only improve data quality, but also push the boundaries of time-lapse studies.

Intuitive software control

Software control is achieved through a fast USB 2.0 connection and the user-friendly LightBridge Graphical User Interface (GUI), where control options include:

pE 800fura lightbridge

              • On/off control
              • LED selection
              • Real time irradiance control
              • Sequence Runner
              • Save and load pre-sets
              • pE-800fura start-up settings
              • Analogue settings
Ideal for optogenetics and electrophysiology

Eight individual analogue inputs allow automated high-speed irradiance control from 0-100% for each channel via analogue signal (0-10 V). This is ideal for many fluorescence applications as well as electrophysiology and optogenetics.

High Irradiance, Low Energy

The laser-free pE-800fura Illumination System builds on award-winning CoolLED technology, with stable and reliable performance and ultra-lower power consumption at 127 W. The sustainability benefits go beyond energy efficiency, and by removing the need for toxic mercury, the pE-800fura is a natural choice for cleaner, greener fluorescence microscopy.

Fit to your microscope

The compact system is compatible with the majority of microscope models. Liquid light guide delivery offers flexible placement.

  • Broad spectrum from 340-635 nm tailored to popular calcium indicators and fluorophores
  • 8 selectable channels offer versatility with any channels selectable simultaneously at 100%
  • Industry-leading <7 µs TTL triggering for high-speed analysis
  • Affordable automation with Sequence Runner and a single TTL-out
  • Removable inline excitation filter holder: no moving parts for high-speed imaging
  • Software , TTL, analogue control options for optimised illumination
  • Optimised irradiance control in 1% steps (2-100%) and fine control from 0-2%
  • Light delivery via liquid Light Guide for maximum compatibility
  • Stable illumination minimises noise for precise calcium analysis and reduced false positives
  • High signal-to-noise for crisp images and quality data with less calcium indicator, reducing toxicity and cost
  • UV optimised optics maximises irradiance for Fura-2 and similar indicators

Plus all the benefits afforded by LED Illumination Systems, such as sustainability and longevity.

The system comprises a pE-800fura Light Source, 8-Position Excitation Filter Holder, filters for Fura-2, and Power Supply.

Control

Graphical user interface (GUI)

CoolLED LightBridge GUI operates via USB to allow:

  • On/off control
  • LED selection
  • Real time irradiance control
  • Sequence Runner
  • Save and load pre-sets
  • pE-800 start-up settings
  • Analogue settings

Download LightBridge GUI 

Triggering speeds dependent on individual PC operating system.

TTL & analogue
8 TTL inputs allow independent on/off control of each channel
8 analogue inputs 0-10 V, 0-50 kHz for dynamic control of irradiance from external analogue signals Global TTL for on/off synchronisation to camera
Triggering speed <7 µs

Sequence Runner
Combining software control with the speed of TTL, the Sequence Runner program allows LED sequences and irradiance to be programmed via LightBridge or compatible imaging software (e.g. NIS Elements). The sequences are controlled via global TTL from a single TTL input such as from a camera TTL out, and therefore requires minimal electronic hardware.

Connectivity:
USB 2 for PC connection. All other TTL and analogue inputs via BNC or SMB.

Light delivery

Light delivery via the standard 3 mm liquid light guide keeps the source of illumination remote from the microscope. Optional pE-UV Universal Collimator and microscope adaptors are available.

Specification

Power requirements
100-240 V a.c. 50/60 Hz, 1.4 A

Power consumption

Maximum power consumption: 127 W

Maximum power consumption when on standby: 6.4 W

Dimensions

pE-800fura Light Source: 173 mm(w) x 247 mm(d) x 174 mm(h) – Weight 3.51 kg
pE-800fura Power Supply: 164 mm(w) x 64 mm(d) x 35 mm(h) – Weight 0.58 kg
pE-UV Universal Collimator: 44 mm(w) x 86 mm(d) x 44 mm(h) – Weight 0.17 kg

Environment & Safety

Adopting LED light sources for fluorescence microscopy helps laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps. CoolLED’s products have the following benefits:

  • Mercury-free and laser-free
  • Energy Efficient: 80% less power
  • Long lifetime
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues
Warranty

Industry-leading 36 months (please note, the 340 nm LED is warranted for 3000 hours accumulated use). Read more here. 

All data correct at time of publication

2107002 pE800fura Spectrum v3 01

We are often asked about the power, intensity or irradiance of CoolLED Illumination Systems. The answer is not as simple as you might believe from many websites and data sheets, and it can be difficult to compare data from these sources as measuring set ups differ.

Photons are also lost as light travels from the light source to sample plane. The only way to objectively compare light sources is by measuring irradiance at the sample plane.  To understand why we use the term irradiance and how to measure and compare light sources with accuracy and precision, download our white paper. Or please contact us if you have further questions.

 

For performance data, please contact us

CoolLED designs and manufactures LED illumination for microscopy and is ideally placed to deliver OEM illumination requirements.
  • Streamlined development using proven off-the-shelf technology
  • Professional expert support
  • Market-leading warranty
  • ‘Try before you buy’ option
  • Trusted experts in LED illumination technology since 2006

The pE-800fura is available in the Amora Series: our proven off-the-shelf product range adapted to offer unparalleled customisation opportunities.

Click the button to find out more.

  • My team told me that the pE-800fura is a very good light source – powerful and suitable for multi-purpose applications. The same for our academic collaborators who clearly saw the difference compared to their aging light source.

    Olivier Chanteux, Sales manager, CEO, Inscoper (pE-800fura)

How to trigger the pE-400<sup>max</sup> in Evident cellSens using the pE-6501

How to trigger the pE-400max in Evident cellSens using the pE-6501

Using LightBridge as a Virtual Control Pod

Using LightBridge as a Virtual Control Pod

CoolLED for Optogenetics

CoolLED for Optogenetics

pE-800 Series LightBridge – Power Up Configurator

pE-800 Series LightBridge – Power Up Configurator

Optical Filters Explained

Optical Filters Explained

pE-800 Series explained

pE-800 Series explained

CoolLED Multi-Wavelength LED Microscopy Illumination

CoolLED Multi-Wavelength LED Microscopy Illumination

Sequence Runner for high-speed imaging

Sequence Runner for high-speed imaging

Setting up the USB to TTL conversion kit

Setting up the USB to TTL conversion kit

Configuring cellSens

Configuring cellSens

Configuring NIS Elements

Configuring NIS Elements

How to use The LightBridge

How to use The LightBridge

pE-800 Series inline excitation filters

pE-800 Series inline excitation filters

Fitting the pE-800 Series to an Olympus IX73

Fitting the pE-800 Series to an Olympus IX73

Fitting the pE-800 Series to a Leica DMi8

Fitting the pE-800 Series to a Leica DMi8

Fitting the pE-800 Series to a Nikon Ti2

Fitting the pE-800 Series to a Nikon Ti2

Configuring Hamamatsu HCImage

Configuring Hamamatsu HCImage

Configuring MetaMorph

Configuring MetaMorph

Configuring µManager

Configuring µManager

CoolLED Optimised filters

CoolLED Optimised filters

Light SourceApplicationTypeCoolLED LEDChromaSemrock
pE-800fura (LLG Fit)FluorescenceSingle550 & 580GYR (525-660)49008-et-mcherry39010 AT Tex. RedYFP-2427BLED-mCherry-A
pE-800fura (LLG Fit)FluorescenceSingle580 & 635GYR (525-660)49006-et-cy539007 AT Cy5Cy5-4040C
pE-800fura (LLG Fit)FluorescenceSingle550GYR (525-660)49004-et-cy349005 ET dsRedTxRed-4040CLED-TRITC-A
pE-800fura (LLG Fit)FluorescenceSingle47046049054-coolled-gfp
pE-800fura (LLG Fit)FluorescenceSingle550 & 580GYR (525-660)49055-coolled-mcherry-149056 CoolLED mCherry #2
pE-800fura (LLG Fit)FluorescenceSingle380365 or 40049053-coolled-dapi
pE-800fura (LLG Fit)FluorescenceSingle550 & 580GYR (525-660)49008-et-mcherry39010 AT Tex. RedYFP-2427BLED-mCherry-A
pE-800fura (LLG Fit)FluorescenceSingle635GYR (525-660)49006-et-cy539007 AT Cy5Cy5-4040C
pE-800fura (LLG Fit)FluorescenceSingle550GYR (525-660)49004-et-cy349005 ET dsRedTxRed-4040CLED-TRITC-A
pE-800fura (LLG Fit)FluorescenceSingle47046049054-coolled-gfp
pE-800fura (LLG Fit)FluorescenceSingle470-50046049002-et-egfp39002 AT eGFPGFP-4050BGFP-A-Basic
pE-800fura (LLG Fit)FluorescenceSingle43546049001-et-ecfp49013 ET TealLED-CFP-ALED-mTFP-A
pE-800fura (LLG Fit)FluorescenceSingle38040049021 eBFP249028 ET Dapi 395LED-DAPI-B
pE-800fura (LLG Fit)FluorescenceMultiband380, 470, 580UV 400, 500, 580DA/FI/TX-B-000
pE-800fura (LLG Fit)FluorescenceSedat380, 435, 500, 580, 635UV 400, 500, 550, 63589903 – ET – BV421/BV480/AF488/AF568/AF647 Quinta Band set
pE-800fura (LLG Fit)FluorescenceMultiband380, 470-500, 550, 635UV 400, 470 & 500, 550, 63589401 – ET – DAPI/FITC/TRITC/CY5 Quad
pE-800fura (LLG Fit)FluorescenceMultiband380, 470, 550UV 400, 470, 550DA/FI/TR-A-000
pE-800fura (LLG Fit)FluorescenceMultiband380, 470-500UV 400, 470DA/FI-A-000
pE-800fura (LLG Fit)FluorescenceSedat340 or 380, 470-500, 580UV 365 or 400, 470, 580LED-DA/FI/TX-3X3M-B-000
pE-800fura (LLG Fit)FluorescenceMultiband340 or 380, 470-500, 580UV 365 or 400, 470, 580LED-DA/FI/TX-A-000
pE-800fura (LLG Fit)FluorescencePinkel340 or 380, 470-500, 580UV 365 or 400, 470, 58069402LED-DA/FI/TX-3X-B-000
pE-800fura (LLG Fit)FluorescenceMultiband380, 470-500, 550, 635UV 365 or 400, 470, 550, 635DA/FI/TR/Cy5-A-000
pE-800fura (LLG Fit)FluorescenceSedat340 or 380, 470-500-550-635UV 365 or 400, 470, 550, 635LED-DA/FI/TR/Cy5-4X-B-000
pE-800fura (LLG Fit)FluorescenceMultiband340 or 380, 470-500-550-635UV 365 or 400, 470, 550, 635LED-DA/FI/TR/Cy5-B-000
pE-800fura (LLG Fit)FluorescenceMultiband380, 470-500, 550, 635UV 365 or 400, 470, 550, 63589402-et-dapi-fitc-tritc-cy5
pE-800fura (LLG Fit)FluorescenceMultiband380, 470, 550 & 580UV 365 or 400, 470, 550 & 58069401-et-triple-multi-led
pE-800fura (LLG Fit)FluorescenceSedat340 or 380, 470-500-550-635UV 365 or 400, 470 & 500, 550, 63589000 – ET – Sedat Quad
pE-800fura (LLG Fit)FluorescencePinkel/Sedat Hybrid340 or 380, 470-500-550-635UV 365 or 400, 470 & 500, 550, 63589404 – ET – 391-32/479-33/554-24/638-31 Multi LED set with single band emitters
pE-800fura (LLG Fit)FluorescencePinkel340 or 380, 470-500-550-635UV 365 or 400, 470 & 500, 550, 63589400-et-dapi-fitc-tritc-cy5
pE-800fura (LLG Fit)FluorescenceSingle49053-coolled-dapiLED-DAPI-B-000
pE-800fura (LLG Fit)FluorescenceSingle380DyLight405-C-000
pE-800fura (LLG Fit)FluorescenceMultiband435, 500, 580LED-CFP/YFP/mCherry-3X3M-A-000
pE-800fura (LLG Fit)RatiometricPinkel340, 38079001 – ET – Fura 2
pE-800fura (LLG Fit)RatiometricPinkel340, 380, 435, 50079002 – ET – Fura 2/BCECF
pE-800fura (LLG Fit)RatiometricPinkel340, 380, 55079003 – ET – Fura 2/TRITC
pE-800fura (LLG Fit)RatiometricPinkel340, 380, 47079004 – ET – Fura 2/GFP
pE-800fura (LLG Fit)RatiometricPinkel435, 50079005 – ET – BCECF
pE-800fura (LLG Fit)RatiometricSingle34079006 – ET – INDO
pE-800fura (LLG Fit)RatiometricSingle50079009 – ET – SNARF with 500nm excitation
pE-800fura (LLG Fit)RatiometricSingle55079010 – ET – SNARF with 550nm excitation
pE-800fura (LLG Fit)Calcium/pHSingle50049003 – ET – EYFP
pE-800fura (LLG Fit)Calcium/pHSingle470 & 50049011 – ET – FITC/Alexa Fluor 488/Fluo3/Oregon GreenSpGr-B-000FITC-5050A-000
pE-800fura (LLG Fit)Calcium/pHSingle470 & 500FITC-3540C-000
pE-800fura (LLG Fit)Calcium/pHSingle5504906349004 ET Cy3LED-TRITC-ASpOr-B
pE-800fura (LLG Fit)Calcium/pHSingle55049305 – ET – Orange#1 FISHCy3-4040C-000
pE-800fura (LLG Fit)Calcium/pHSingle550 & 58049055-coolled-mcherry-149008-et-mcherrymCherry-C-000LED-mCherry-A-000
pE-800fura (LLG Fit)Calcium/pHSingle47049002-et-egfp
pE-800fura (LLG Fit)Calcium/pHSingle500 & 55049023 – ET – AlexaFluor 514/ZsYellow/EosiLED-YFP-A
pE-800fura (LLG Fit)FluorescenceSingle38039000 AT Dapi49028 ET Dapi 395LED-DAPI-B
pE-800fura (LLG Fit)FluorescencePinkel340, 380T400lp & ET510/80mFF409-Di03FF01-510/84
pE-800fura (LLG Fit)FluorescencePinkel340, 380, White (BLU/GRN)T495lpxru, ET470/40x, ET535/70m
pE-800fura (LLG Fit)FluorescencePinkel340, 380, White (BLU/GRN)79003bs, 79003m, ET555/25x
pE-800-FR-L-SYS-ZZ: pE-800fura Fluorescence Illumination System. For use with 3 mm Liquid Light Guide. Includes Light Source, Power Supply, Excitation Filter Holder (holds up to 8 filters), Excitation filters for 340 nm & 380 nm, USB cable & ZZ Plug
pE-1906: 1.5 m long, 3 mm diameter liquid light guide
pE-1908: 3 m long, 3 mm diameter liquid light guide
pE-340-FR-COLL-YYY: pE-UV Universal Collimator & customer specified adaptor.
pE-CABLE-9WBNC-FR: BNC Breakout Cable – 15 pin male connector to 9 x BNC female. Suitable for analogue or digital
pE-CABLE-9WSMB-FR: SMB Breakout Cable – 15 pin male connector to 9 x SMB female. Suitable for analogue or digital
pE-6501-8-FR: USB-TTL Conversion Kit for TTL control of pE-800fura with additional BNC for other peripherals (e.g. pT-100 for transmitted light). Connects to PC via USB and is compatible with range of imaging software.
pE-6501-8-FRS: USB-TTL Conversion Kit when purchased with a pE-800fura Illumination System
pE-CABLE-15WHD2M: Nikon communication cable for NiDAQ

To specify microscope code (YYY), see Adaptors

To specify Power Cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

USB-TTL Conversion kit

USB-TTL Conversion kit

Breakout cables

Breakout cables

Liquid Light Guide

Liquid Light Guide

pE-UV Universal Collimator

pE-UV Universal Collimator

We are working to fully integrate the pE-800 into all major third party imaging software programs, but in the meantime you can use the pE-6501-8 USB controlled TTL trigger box for software control. Tutorial videos show you how to configure cellSens and MetaMorph.

The CoolLED pE Driver is first required for software integration, and can be downloaded here.

CompanySoftwareSummaryQuick Start GuideVideo
HamamatsuHCImage
InscoperInscoper
Intelligent Imaging Innovations (3i)SlideBook 
µManagerµManager
Molecular DevicesMetaMorph
NikonNIS Elements
Olympus/EvidentcellSens

Contact CoolLED for further information.