pE 800 LLG SQ

Overview

The arrival of the pE-800 sees LEDs take centre stage as the widefield illumination method of choice. Featuring eight individually controllable LEDs and lightning fast TTL switching, the pE-800 delivers the highest quality data with minimum total cost of ownership. Versatile, intuitive and backed by CoolLED’s world-renowned support and a 36-month warranty, the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.

pE 800 Spectrum Graph 72dpi
Mix & match with eight channels

Eight powerful and efficient LEDs offer broad spectral coverage from 365-740 nm for use with fluorophores ranging from DAPI to Cy7. The choice of fluorophore is no longer limited by the light source, and choosing the optimal set for each application has never been easier – even as requirements evolve over time.

All eight channels can be individually controlled, with each LED selected to perfectly match the selected fluorophores. Excitation filters with white light sources let through more unwanted light, and by matching the LED this improves the signal to noise ratio for contrast-rich images revealing finer details and more information.

Capture high-speed events

Industry-leading <7 µs TTL triggering is a breakthrough for live cell imaging. Capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8  (USB controlled TTL Conversion Kit).

TTL triggering graph

The ability to fit inline excitation filters capitalises on TTL trigger speeds, as single-band excitation filters can be installed for each channel. When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for expensive external filter wheels and overcomes the latency restrictions of mechanical movement, presenting a low-cost approach to high-speed imaging.

pE 800 Excitation filter holder 1

Affordable automation with Sequence Runner

The popular Sequence Runner program takes speed one step further and combines the versatility of software with the <7 µs speeds of TTL, with minimal requirement for expensive electronic hardware. Once an LED and irradiance sequence are specified in software, Sequence Runner provides lightning fast synchronised triggering via the global TTL-in of the pE-800 and a single TTL-out from a camera (or other hardware).

The pE-800 is unique in offering the only 8-channel Sequence Runner available, and when combined with inline filters, transforms a manual microscope into an affordable and powerful eight-channel automated imaging system. As laboratory budgets become stretched, the pE-800 presents the ideal cost-effective Illumination System which makes high-end LED technology more accessible to life science researchers.

Protecting your samples

Tight hardware synchronisation not only increases temporal resolution, but also means samples are exposed only during acquisition, protecting against photobleaching and phototoxicity and pushing the boundaries of time-lapse studies.

In the Lightbridge graphical user interface (see below), live samples can be further protected by balancing the irradiance to the lowest level possible while still maintaining image quality, with fine (0,1 %) control from 0-2 %, and in 1 % steps up to 100 %. And the more life-like a cell behaves, the more valuable a data set becomes.

Intuitive software control

Software control is achieved through a fast USB 2.0 connection and the user-friendly LightBridge Graphical User Interface (GUI), where control options include:

2009011 GUI Mockup v2

              • On/off control
              • LED selection
              • Real time irradiance control
              • Sequence Runner
              • Save and load pre-sets
              • pE-800 start-up settings
              • Analogue settings

Users can also benefit from full software integration in Nikon NIS Elements.

Beyond imaging

Eight individual analogue inputs allow irradiance to be controlled from 0-100 % for each channel via analogue signal (0-10 v), which is ideal for electrophysiology and optogenetics applications.

Fit to your microscope

The compact system is compatible with the majority of microscope models. Liquid light guide delivery offers flexible placement.

Sustainable illumination

The compact pE-800 Illumination System builds on award-winning CoolLED technology, with stable and reliable performance and ultra-low power consumption. The sustainability benefits go beyond energy efficiency, and by removing the need for toxic mercury, the pE-800 is a natural choice for cleaner, greener labs.

  • 8 selectable channels offers versatility with any channels selectable simultaneously at 100 %
  • Individual control of selected LED wavelengths reduces background
  • Broad spectrum from 365-740 nm, covering DAPI-Cy7
  • Industry-leading <7 µs TTL triggering for high-speed imaging
  • Sequence Runner for software control with speed of TTL
  • Removable inline excitation filter holders: no moving parts for high-speed imaging
  • Software, TTL, analogue control options for optimised illumination
  • Optimised irradiance control in 1 % steps (2-100 %) and fine control from 0-2 %
  • Light delivery via liquid Light Guide for maximum compatibility

Plus all the benefits afforded by LED Illumination Systems, such as sustainability and longevity.

The system comprises a pE-800 Light Source, 8-Position Excitation Filter Holder and Power Supply.

Control

Graphical user interface (GUI)

CoolLED LightBridge GUI operates via USB to allow:

  • On/off control
  • LED selection
  • Real time irradiance control
  • Sequence Runner
  • Save and load pre-sets
  • pE-800 start-up settings
  • Analogue settings

Triggering speeds dependent on individual PC operating system.

TTL & analogue
8 TTL inputs allow independent on/off control of each channel
8 analogue inputs 0-10 V, 0-50 kHz for dynamic control of irradiance from external analogue signals Global TTL for on/off synchronisation to camera
Triggering speed <7 µs

Sequence Runner
Combining software control with the speed of TTL, the Sequence Runner program allows LED sequences and irradiance to be programmed via LightBridge. The sequences are controlled via global TTL from a single TTL input such as from a camera TTL out or other external hardware, and therefore requires minimal electronic hardware.

Connectivity:
USB 2 for PC connection. All other TTL and analogue inputs via BNC or SMB.

Light delivery

Light delivery via the standard 3 mm liquid light guide keeps the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can also be selected.

Choose 365 nm or 400 nm variant for UV

For optimum DAPI excitation, the pE-800 is available with a 365 nm or 400 nm LED for compatibility with existing filter sets. Find out more in our tutorial video here.

Specification

Power requirements
100-240 V a.c. 50/60 Hz, 1.4 A

Power consumption

Maximum power consumption: 127 W

Maximum power consumption when on standby: 6.4 W

Dimensions

pE-800 Light Source: 173 mm(w) x 247 mm(d) x 174 mm(h) – Weight 3.51 kg
pE-800 Power Supply: 164 mm(w) x 64 mm(d) x 35 mm(h) – Weight 0.58 kg

pE-Universal Collimator: 44 mm(w) x 86 mm(d) x 44 mm(h) – Weight 0.17 kg

Environment & Safety

Adopting LED light sources for fluorescence microscopy helps laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps. CoolLED’s products have the following benefits:

  • Mercury-free and laser-free
  • Energy Efficient
  • Long lifetime
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues
Warranty

Industry-leading 36 months. Read more here. 

 

All data correct at time of publication

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How to use The LightBridge

How to use The LightBridge

Choosing an SB or MB model

Choosing an SB or MB model

pE-800 inline excitation filters

pE-800 inline excitation filters

Fitting the pE-800 to an Olympus IX73

Fitting the pE-800 to an Olympus IX73

Fitting the pE-800 to a Leica DMi8

Fitting the pE-800 to a Leica DMi8

Light SourceEmission ColourTypeCoolLED LEDChromaSemrock
pE-800Blue-Teal-Orange-Red-IRSedatUV 400, 500, 550, 63589903
pE-800Blue-Green-Orange-Far RedSedatUV 365 or 400, 470 & 500, 550, 63589000
pE-800Blue-Green-Orange-Far Red-IRSedatUV 365 or 400, 470, 550, 635LED-DA/FI/TR/Cy5/Cy7-5X5M-A-000
pE-800Blue-Green-Orange-Far RedSedatUV 365 or 400, 470, 550, 635LED-DA/FI/TR/Cy5-4X4M-B-000
pE-800Blue-Green-RedSedatUV 365 or 400, 470, 580LED-DA/FI/TX-3X3M-B-000
pE-800Blue-Green-RedSedatUV 400, 470 & 500, 58089014LED-CFP/YFP/mCherry-3X3M-A-000
pE-800Blue-Green-OrangeSedatUV 400, 470 & 500, 55089013
pE-800Cyan-Teal-OrangeSedat435, 500, 55089007
pE-800Cyan-Teal-RedSedat435, 500, 58089006
pE-800Green-Orange-Far RedSedat470, 550, 63589016
pE-800Green-OrangeSedat470, 55089019
pE-800Green-RedSedat470, 58089021
pE-800Green-RedSedat470 & 500, 58089017
pE-800Cyan-TealSedat435, 50089002
pE-800Orange-Far RedSedat550, 63589022
pE-800Blue-Green-Orange-Far RedPinkel/Sedat HybridUV 365 or 400, 470 & 500, 550, 63589404
pE-800Cyan-Teal-Red-IRPinkel/Sedat Hybrid435, 500, 580, 74089405
pE-800Blue-Green-Orange-Far RedPinkelUV 365 or 400, 470 & 500, 550, 63589400
pE-800Blue-Green-Orange-Far RedPinkelUV 365 or 400, 470, 550, 635LED-DA/FI/TR/Cy5/Cy7-5X-A-000LED-DA/FI/TR/Cy5-4X-B-000
pE-800Blue-Green-RedPinkelUV 365 or 400, 470, 58069402LED-DA/FI/TX-3X-B-000
pE-800Cyan, Green, RedPinkel435, 500, 58069308LED-CFP/YFP/mCherry-3X-A-000
pE-800Blue-Green-RedPinkelUV 400, 470 & 500, 58069302
pE-800Blue-Green-Orange-Far Red-IRMultibandUV 365 or 400, 470, 550, 635, 740LED-DA/FI/TR/Cy5/Cy7-A-000
pE-800Blue-Green-Orange-Far RedMultibandUV 365 or 400, 470, 550, 635LED-DA/FI/TR/Cy5-B-000
pE-800Blue-Green-Orange-Far RedMultibandUV 365 or 400, 470, 550, 635DA/FI/TR/Cy5-A-000
pE-800Blue-Green-Orange-Far RedMultibandUV 365 or 400, 470, 550, 63589402
pE-800Blue-Green-Orange-Far RedMultibandUV 400, 470 & 500, 550, 63589401
pE-800Cyan-Green-Red-IRMultiband435, 500, 580, 74089403
pE-800Blue-Green-RedMultibandUV 365 or 400, 470, 580LED-DA/FI/TX-A-000
pE-800Blue-Green-RedMultibandUV 365 or 400, 470, 550 & 58069401
pE-800Blue-Green-OrangeMultibandUV 400, 470, 550DA/FI/TR-A-000
pE-800Blue-Green-RedMultibandUV 400, 500, 580DA/FI/TX-B-000
pE-800Cyan-Teal-RedMultiband435, 500, 580LED-CFP/YFP/mCHerry-A-000
pE-800Green-RedMultiband470, 550 & 58059022
pE-800Cyan-Green-Red-IRMultiband435, 50059017DA/FI-A-000
pE-800Blue-GreenMultibandUV 400, 470DA/FI-A-000
pE-800Green-RedMultiband470, 580FITC/TxRed-A-000
pE-800Green-OrangeMultiband470, 550GFP/DsRed-A-000
pE-800BlueSingleUV 365 or 40049053LED-DAPI-B-000
pE-800BlueSingle365DyLight405-C-000
pE-800CyanSingle43549001LED-mTFP-A-000
pE-800CyanSingle435LED-CFP-A-000
pE-800CyanSingle435LuciferYellow-C-000
pE-800Cyan-GreenSingle435, 47049013
pE-800GreenSingle47049054GFP-4050B-000
pE-800GreenSingle47049061FITC-5050A-000
pE-800GreenSingle47049002GFP-3035D-000
pE-800GreenSingle470LED-FITC-A-000
pE-800GreenSingle470FITC-3540C-000
pE-800GreenSingle470FITC-2024B-000
pE-800GreenSingle470, 500SpGr-B-000
pE-800GreenSingle470, 50049011
pE-800GreenSingle47049020
pE-800Green-YellowSingle470, 50049023
pE-800GreenSingle50049003YFP-2427B-000
pE-800GreenSingle500, 550LED-Venus-A-000
pE-800OrangeSingle55049014Cy3-4040C-000
pE-800Orange-RedSingle55049062LED-TRITC-A-000
pE-800RedSingle550, 58049056Cy3.5-A-Basic-000
pE-800RedSingle550, 58049055mCherry-C-000
pE-800Orange-RedSingle550, 58049031TxRed-4040C-000
pE-800RedSingle580LED-mCherry-A-000
pE-800Far Red-NIRSingle580, 63549015Cy5-4040C-000
pE-800Far Red-NIRSingle580, 63549006
pE-800Far Red-NIRSingle63549009LED-Cy5-A-000
pE-800Far Red-NIRSingle63549022Cy5.5-C-000
pE-800NIRSingle635, 74049007
pE-800NIRSingle74049037Cy7-B-000
pE-800NIRSingle74049030LED-Cy7-A-000
pE-800NIRSingle740IRDYE800-33LP-A-000
pE-800NIRSingle740ICG-B-000
pE-800-L-SB-SYS-ZZ: pE-800 Fluorescence Illumination System. SB (365 nm). For use with 3 mm Liquid Light Guide. Includes Light Source, Power Supply, Excitation Filter Holder (holds up to 8 filters) & USB cable
pE-800-L-MB-SYS-ZZ: pE-800 Fluorescence Illumination System. MB (400 nm). For use with 3 mm Liquid Light Guide. Includes Light Source, Power Supply, Excitation Filter Holder (holds up to 8 filters) & USB cable
pE-1906: 1.5 m long, 3 mm diameter liquid light guide
pE-1908: 3 m long, 3 mm diameter liquid light guide
pE-10400-YYY: pE-Universal collimator & customer specified adaptor
pE-CABLE-9WBNC: BNC Breakout Cable – 15 pin male connector to 9 x BNC female. Suitable for analogue or digital
pE-CABLE-9WSMB: SMB Breakout Cable – 15 pin male connector to 9 x SMB female. Suitable for analogue or digital
pE-6501-8: USB-TTL Conversion Kit
pE-6501-8S: USB-TTL Conversion Kit when purchased with a pE-800 Illumination System

To specify microscope code (YYY), see Adaptors

To specify Power Cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

pE-Universal Collimator

pE-Universal Collimator

Breakout cables

Breakout cables

Liquid Light Guide

Liquid Light Guide

pE-6501-8

pE-6501-8

The CoolLED pE Driver is first required for software integration, and can be downloaded here.

CompanySoftwareSummaryScreenshotQuick Start GuideVideo
NikonNIS Elements

CoolLED Proprietary ZEN Bridge software is available – Contact CoolLED for further information.