Overview
The arrival of the pE-800 sees LEDs take centre stage as the widefield illumination method of choice. Featuring eight individually controllable LEDs and lightning fast TTL switching, the pE-800 delivers the highest quality data with minimum total cost of ownership. Versatile, intuitive and backed by CoolLED’s world-renowned support and a 36-month warranty, the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.
Mix & match with eight channels
Eight powerful and efficient LEDs offer broad spectral coverage from 365-740 nm for use with fluorophores ranging from DAPI to Cy7. The choice of fluorophore is no longer limited by the light source, and choosing the optimal set for each application has never been easier – even as requirements evolve over time.
All eight channels can be individually controlled, with each LED selected to perfectly match the selected fluorophores. Excitation filters with white light sources let through more unwanted light, and by matching the LED this improves the signal to noise ratio for contrast-rich images revealing finer details and more information.
Capture high-speed events
Industry-leading <7 µs TTL triggering is a breakthrough for live cell imaging. Capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8 (USB controlled TTL Conversion Kit).
The ability to fit inline excitation filters capitalises on TTL trigger speeds, as single-band excitation filters can be installed for each channel. When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for expensive external filter wheels and overcomes the latency restrictions of mechanical movement, presenting a low-cost approach to high-speed imaging.
Affordable automation with Sequence Runner
The popular Sequence Runner program takes speed one step further and combines the versatility of software with the <7 µs speeds of TTL, with minimal requirement for expensive electronic hardware. Once an LED and irradiance sequence are specified in software, Sequence Runner provides lightning fast synchronised triggering via the global TTL-in of the pE-800 and a single TTL-out from a camera (or other hardware).
The pE-800 is unique in offering the only 8-channel Sequence Runner available, and when combined with inline filters, transforms a manual microscope into an affordable and powerful eight-channel automated imaging system. As laboratory budgets become stretched, the pE-800 presents the ideal cost-effective Illumination System which makes high-end LED technology more accessible to life science researchers.
Protecting your samples
Tight hardware synchronisation not only increases temporal resolution, but also means samples are exposed only during acquisition, protecting against photobleaching and phototoxicity and pushing the boundaries of time-lapse studies.
In the Lightbridge graphical user interface (see below), live samples can be further protected by balancing the irradiance to the lowest level possible while still maintaining image quality, with fine (0,1 %) control from 0-2 %, and in 1 % steps up to 100 %. And the more life-like a cell behaves, the more valuable a data set becomes.
Intuitive software control
Software control is achieved through a fast USB 2.0 connection and the user-friendly LightBridge Graphical User Interface (GUI), where control options include:
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- On/off control
- LED selection
- Real time irradiance control
- Sequence Runner
- Save and load pre-sets
- pE-800 start-up settings
- Analogue settings
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It is important to note that The LightBridge is only compatible with the pE-800 Series. In addition to The LightBridge, which can be downloaded here, users can also benefit from full integration into third party imaging software.
Beyond imaging
Eight individual analogue inputs allow irradiance to be controlled from 0-100 % for each channel via analogue signal (0-10 v), which is ideal for electrophysiology and optogenetics applications.
Fit to your microscope
The compact system is compatible with the majority of microscope models. Liquid light guide delivery offers flexible placement.
Sustainable illumination
The compact pE-800 Illumination System builds on award-winning CoolLED technology, with stable and reliable performance and ultra-low power consumption. The sustainability benefits go beyond energy efficiency, and by removing the need for toxic mercury, the pE-800 is a natural choice for cleaner, greener labs.
- 8 selectable channels offers versatility with any channels selectable simultaneously at 100 %
- Individual control of selected LED wavelengths reduces background noise
- Broad spectrum from 365-740 nm, covering DAPI-Cy7
- Industry-leading <7 µs TTL triggering for high-speed imaging
- Sequence Runner for software control with speed of TTL
- Removable inline excitation filter holders: no moving parts for high-speed imaging
- Software, TTL, analogue control options for optimised illumination
- Optimised irradiance control in 1 % steps (2-100 %) and fine control from 0-2 %
- Light delivery via liquid Light Guide for maximum compatibility
Plus all the benefits afforded by LED Illumination Systems, such as sustainability and longevity.
The system comprises a pE-800 Light Source, 8-Position Excitation Filter Holder and Power Supply.
Control
Graphical user interface (GUI)
CoolLED LightBridge GUI operates via USB to allow:
- On/off control
- LED selection
- Real time irradiance control
- Sequence Runner
- Save and load pre-sets
- pE-800 start-up settings
- Analogue settings
Triggering speeds dependent on individual PC operating system.
TTL & analogue
8 TTL inputs allow independent on/off control of each channel
8 analogue inputs 0-10 V, 0-50 kHz for dynamic control of irradiance from external analogue signals Global TTL for on/off synchronisation to camera
Triggering speed <7 µs
Sequence Runner
Combining software control with the speed of TTL, the Sequence Runner program allows LED sequences and irradiance to be programmed via LightBridge. The sequences are controlled via global TTL from a single TTL input such as from a camera TTL out or other external hardware, and therefore requires minimal electronic hardware.
Connectivity:
USB 2 for PC connection. All other TTL and analogue inputs via BNC or SMB.
Light delivery
Light delivery via the standard 3 mm liquid light guide keeps the source of illumination remote from the microscope. An optional pE-Universal Collimator and microscope adaptor can also be selected.
Choose 365 nm or 400 nm variant for UV
For optimum DAPI excitation, the pE-800 is available with a 365 nm or 400 nm LED for compatibility with existing filter sets. Find out more in our tutorial video here.
Specification
Power requirements
100-240 V a.c. 50/60 Hz
Power consumption
Maximum power consumption: 127 W
Maximum power consumption when on standby: 6.4 W
Dimensions
pE-800 Light Source: 173 mm(w) x 247 mm(d) x 174 mm(h) – Weight 3.51 kg
pE-800 Power Supply: 164 mm(w) x 64 mm(d) x 35 mm(h) – Weight 0.58 kg
pE-Universal Collimator: 44 mm(w) x 86 mm(d) x 44 mm(h) – Weight 0.17 kg
Environment & Safety
Adopting LED light sources for fluorescence microscopy helps laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps. CoolLED’s products have the following benefits:
- Mercury-free and laser-free
- Energy Efficient
- Long lifetime
- No bulb replacements
- Reduced risk of eye damage
- Quiet operation
- No special disposal regulations or issues
Warranty
Industry-leading 36 months. Read more here.
All data correct at time of publication
We are often asked about the power, intensity or irradiance of CoolLED Illumination Systems. The answer is not as simple as you might believe from many websites and data sheets, and it can be difficult to compare data from these sources as measuring set ups differ.
Photons are also lost as light travels from the light source to sample plane. The only way to objectively compare light sources is by measuring irradiance at the sample plane. To understand why we use the term irradiance and how to measure and compare light sources with accuracy and precision, download our white paper. Or please contact us if you have further questions.
For performance data, please contact us
CoolLED designs and manufactures LED illumination for microscopy and is ideally placed to deliver OEM illumination requirements.
- Streamlined development using proven off-the-shelf technology
- Professional expert support
- Market-leading warranty
- ‘Try before you buy’ option
- Trusted experts in LED illumination technology since 2006
The pE-800 is available in the Amora Series: our proven off-the-shelf product range adapted to offer unparalleled customisation opportunities.
Click the button to find out more.
I’m always surprised at the brightness of LED light sources these days. Most people think that the brighter the excitation light is, the brighter the signal will be, and they are used to setting the irradiance to 25, 50 or even 100%. But for both the pE-300ultra and the pE-800, I constantly need to recommend starting at 1 or 2%, which will produce plenty of signal, and can always be increased.
Sam Duwé, Microscopy Specialist, Advanced Optical Microscopy Centre, Hasselt University, Belgium (pE-300ultra)
Spinal Cord labelled with DAPI/EGFP-Lamnin-GFAP (pE-800)
Enteric Glia Cells (Green) and Endothelial Cells (Red), acquired using the CoolLED (pE-800)
Enteric Nervous System labelled with GFAP and HuCD (pE-800)
Primary neuron from a rat cortex – supplied by Transnetyx Tissue by BrainBits (CoolLED pE-800)
Bovine pulmonary epithelial cells. (CoolLED pE-800)
How to trigger the pE-400max in Evident cellSens using the pE-6501
Using LightBridge as a Virtual Control Pod
CoolLED for Optogenetics
Sequence Runner for Nikon NIS Elements
pE-800 Series LightBridge – Power Up Configurator
Sequence Runner: Cost-effective automation
Optical Filters Explained
pE-800 Series explained
CoolLED Multi-Wavelength LED Microscopy Illumination
Sequence Runner for high-speed imaging
Setting up the USB to TTL conversion kit
Configuring cellSens
Configuring NIS Elements
How to use The LightBridge
Choosing an SB or MB model
pE-800 Series inline excitation filters
Fitting the pE-800 Series to an Olympus IX73
Fitting the pE-800 Series to a Leica DMi8
Fitting the pE-800 Series to a Nikon Ti2
Configuring Hamamatsu HCImage
Configuring MetaMorph
Configuring µManager
CoolLED Optimised filters
| Light Source | Application | Type | CoolLED LED | Chroma | Semrock | |||
|---|---|---|---|---|---|---|---|---|
| pE-800 | Fluorescence | Sedat | UV 400, 500, 550, 635 | 89903 – ET – BV421/BV480/AF488/AF568/AF647 Quinta Band set | ||||
| pE-800 | Fluorescence | Sedat | UV 365 or 400, 470 & 500, 550, 635 | 89000 – ET – Sedat Quad | ||||
| pE-800 | Fluorescence | Sedat | UV 365 or 400, 470, 550, 635 | LED-DA/FI/TR/Cy5/Cy7-5X5M-A-000 | ||||
| pE-800 | Fluorescence | Sedat | UV 365 or 400, 470, 550, 635 | LED-DA/FI/TR/Cy5-4X4M-B-000 | ||||
| pE-800 | Fluorescence | Sedat | UV 365 or 400, 470, 580 | LED-DA/FI/TX-3X3M-B-000 | ||||
| pE-800 | Fluorescence | Sedat | UV 400, 470 & 500, 580 | 89014 – ET – DAPI/FITC/Texas Red® | LED-CFP/YFP/mCherry-3X3M-A-000 | |||
| pE-800 | Fluorescence | Sedat | UV 400, 470 & 500, 550 | 89013 – ET – DAPI/FITC/TRITC | ||||
| pE-800 | Fluorescence | Sedat | 435, 500, 550 | 89007 – ET – ECFP/EYFP/DsRed | ||||
| pE-800 | Fluorescence | Sedat | 435, 500, 580 | 89006 – ET – ECFP/EYFP/mCherry | ||||
| pE-800 | Fluorescence | Sedat | 470, 550, 635 | 89016 – ET – FITC/Cy3/Cy5 | ||||
| pE-800 | Fluorescence | Sedat | 470, 550 | 89019 – ET – FITC/TRITC | ||||
| pE-800 | Fluorescence | Sedat | 470, 580 | 89021 – ET – EGFP/mCherry | ||||
| pE-800 | Fluorescence | Sedat | 470 & 500, 580 | 89017 – ET – FITC/Texas Red® | ||||
| pE-800 | Fluorescence | Sedat | 435, 500 | 89002 – ET – ECFP/EYFP | ||||
| pE-800 | Fluorescence | Sedat | 550, 635 | 89022 – ET – Cy3/Cy5 | ||||
| pE-800 | Fluorescence | Pinkel/Sedat Hybrid | UV 365 or 400, 470 & 500, 550, 635 | 89404 – ET – 391-32/479-33/554-24/638-31 Multi LED set with single band emitters | ||||
| pE-800 | Fluorescence | Pinkel/Sedat Hybrid | 435, 500, 580, 740 | 89405 – ET – 436-28/506-21/578-24/730-40 Multi LED set with single band emitters | ||||
| pE-800 | Fluorescence | Pinkel | UV 365 or 400, 470 & 500, 550, 635 | 89400-et-dapi-fitc-tritc-cy5 | ||||
| pE-800 | Fluorescence | Pinkel | UV 365 or 400, 470, 550, 635 | LED-DA/FI/TR/Cy5/Cy7-5X-A-000 | LED-DA/FI/TR/Cy5-4X-B-000 | |||
| pE-800 | Fluorescence | Pinkel | UV 365 or 400, 470, 580 | 69402 | LED-DA/FI/TX-3X-B-000 | |||
| pE-800 | Fluorescence | Pinkel | 435, 500, 580 | 69308-et-ecfp-eyfp-mcherry | LED-CFP/YFP/mCherry-3X-A-000 | |||
| pE-800 | Fluorescence | Pinkel | UV 400, 470 & 500, 580 | 69302-et-dapi-fitc-texas-red | ||||
| pE-800 | Fluorescence | Multiband | UV 365 or 400, 470, 550, 635, 740 | LED-DA/FI/TR/Cy5/Cy7-A-000 | ||||
| pE-800 | Fluorescence | Multiband | UV 365 or 400, 470, 550, 635 | LED-DA/FI/TR/Cy5-B-000 | ||||
| pE-800 | Fluorescence | Multiband | UV 365 or 400, 470, 550, 635 | DA/FI/TR/Cy5-A-000 | ||||
| pE-800 | Fluorescence | Multiband | UV 365 or 400, 470, 550, 635 | 89402-et-dapi-fitc-tritc-cy5 | ||||
| pE-800 | Fluorescence | Multiband | UV 400, 470 & 500, 550, 635 | 89401 – ET – DAPI/FITC/TRITC/CY5 Quad | ||||
| pE-800 | Fluorescence | Multiband | 435, 500, 580, 740 | 89403 – ET – 436-28/506-21/578-24/730-40 Multi LED set | ||||
| pE-800 | Fluorescence | Multiband | UV 365 or 400, 470, 580 | LED-DA/FI/TX-A-000 | ||||
| pE-800 | Fluorescence | Multiband | UV 365 or 400, 470, 550 & 580 | 69401-et-triple-multi-led | ||||
| pE-800 | Fluorescence | Multiband | UV 400, 470, 550 | DA/FI/TR-A-000 | ||||
| pE-800 | Fluorescence | Multiband | UV 400, 500, 580 | DA/FI/TX-B-000 | ||||
| pE-800 | Fluorescence | Multiband | 435, 500, 580 | LED-CFP/YFP/mCHerry-A-000 | ||||
| pE-800 | Fluorescence | Multiband | 470, 550 & 580 | 59022-et-egfp-mcherry | ||||
| pE-800 | Fluorescence | Multiband | 435, 500 | 59017-et-ecfp-eyfp | DA/FI-A-000 | |||
| pE-800 | Fluorescence | Multiband | UV 400, 470 | DA/FI-A-000 | ||||
| pE-800 | Fluorescence | Multiband | 470, 580 | FITC/TxRed-A-000 | ||||
| pE-800 | Fluorescence | Multiband | 470, 550 | GFP/DsRed-A-000 | ||||
| pE-800 | Fluorescence | Single | UV 365 or 400 | 49053-coolled-dapi | LED-DAPI-B-000 | |||
| pE-800 | Fluorescence | Single | 365 | DyLight405-C-000 | ||||
| pE-800 | Fluorescence | Single | 435 | 49001-et-ecfp | LED-mTFP-A-000 | |||
| pE-800 | Fluorescence | Single | 435 | LED-CFP-A-000 | ||||
| pE-800 | Fluorescence | Single | 435 | LuciferYellow-C-000 | ||||
| pE-800 | Fluorescence | Single | 435, 470 | 49013 – ET – TFP (Teal FP) | ||||
| pE-800 | Fluorescence | Single | 470 | 49054-coolled-gfp | GFP-4050B-000 | |||
| pE-800 | Fluorescence | Single | 470 | 49061 | FITC-5050A-000 | |||
| pE-800 | Fluorescence | Single | 470 | 49002-et-egfp | GFP-3035D-000 | |||
| pE-800 | Fluorescence | Single | 470 | LED-FITC-A-000 | ||||
| pE-800 | Fluorescence | Single | 470 | FITC-3540C-000 | ||||
| pE-800 | Fluorescence | Single | 470 | FITC-2024B-000 | ||||
| pE-800 | Fluorescence | Single | 470, 500 | SpGr-B-000 | ||||
| pE-800 | Fluorescence | Single | 470, 500 | 49011 – ET – FITC/Alexa Fluor 488/Fluo3/Oregon Green | ||||
| pE-800 | Fluorescence | Single | 470 | 49020 – ET – Narrow Band EGFP to Minimize Autofluorescence | ||||
| pE-800 | Fluorescence | Single | 470, 500 | 49023 – ET – AlexaFluor 514/ZsYellow/Eosi | ||||
| pE-800 | Fluorescence | Single | 500 | 49003 – ET – EYFP | YFP-2427B-000 | |||
| pE-800 | Fluorescence | Single | 500, 550 | LED-Venus-A-000 | ||||
| pE-800 | Fluorescence | Single | 550 | 49014 – ET – mKO/mOrange | Cy3-4040C-000 | |||
| pE-800 | Fluorescence | Single | 550 | 49062 | LED-TRITC-A-000 | |||
| pE-800 | Fluorescence | Single | 550, 580 | 49056 – ET – Narrow mCherry/Texas Red for 540-580nm LEDs | Cy3.5-A-Basic-000 | |||
| pE-800 | Fluorescence | Single | 550, 580 | 49055-coolled-mcherry-1 | mCherry-C-000 | |||
| pE-800 | Fluorescence | Single | 550, 580 | 49031 – ET – Alexa Fluor 568, Cy3.5 | TxRed-4040C-000 | |||
| pE-800 | Fluorescence | Single | 580 | LED-mCherry-A-000 | ||||
| pE-800 | Fluorescence | Single | 580, 635 | 49015 – ET – AlexaFluor 633 | Cy5-4040C-000 | |||
| pE-800 | Fluorescence | Single | 580, 635 | 49006-et-cy5 | ||||
| pE-800 | Fluorescence | Single | 635 | 49009 – ET – Cy5 Narrow Excitation | LED-Cy5-A-000 | |||
| pE-800 | Fluorescence | Single | 635 | 49022-et-cy5-5 | Cy5.5-C-000 | |||
| pE-800 | Fluorescence | Single | 635, 740 | 49007-et-cy7 | ||||
| pE-800 | Fluorescence | Single | 740 | 49037 – ET – Li_Cor for IR dye 800 | Cy7-B-000 | |||
| pE-800 | Fluorescence | Single | 740 | 49030-et-indocy-gr | LED-Cy7-A-000 | |||
| pE-800 | Fluorescence | Single | 740 | IRDYE800-33LP-A-000 | ||||
| pE-800 | Fluorescence | Single | 740 | ICG-B-000 | ||||
| pE-800-L-SB-SYS-ZZ: | pE-800 Fluorescence Illumination System. SB (365 nm). For use with 3 mm Liquid Light Guide. Includes Light Source, Power Supply, Excitation Filter Holder (holds up to 8 filters) & USB cable |
| pE-800-L-MB-SYS-ZZ: | pE-800 Fluorescence Illumination System. MB (400 nm). For use with 3 mm Liquid Light Guide. Includes Light Source, Power Supply, Excitation Filter Holder (holds up to 8 filters) & USB cable |
| pE-1906: | 1.5 m long, 3 mm diameter liquid light guide |
| pE-1908: | 3 m long, 3 mm diameter liquid light guide |
| pE-10400-YYY: | pE-Universal collimator & customer specified adaptor |
| pE-CABLE-9WBNC: | BNC Breakout Cable – 15 pin male connector to 9 x BNC female. Suitable for analogue or digital |
| pE-CABLE-9WSMB: | SMB Breakout Cable – 15 pin male connector to 9 x SMB female. Suitable for analogue or digital |
| pE-6501-8: | USB-TTL Conversion Kit |
| pE-6501-8S: | USB-TTL Conversion Kit when purchased with a pE-800 Illumination System |
To specify microscope code (YYY), see Adaptors
To specify Power Cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA
- CoolLED pE-800 Series Leaflet
- CoolLED pE-800 Series Quick Start Guide
- CoolLED pE-800 Series Quick Start Guide Spanish
- CoolLED pE-800 Series Quick Start Guide – Portuguese
- CoolLED pE-800 Series User Manual
- CoolLED pE-800 Troubleshooting Guide
- CoolLED pE-800 Series Exploded Diagram
- CoolLED pE-800 Series Declaration of Conformity
- CellSens Set-up Guide for pE-800 with pE-6501-8
- uManager Set-up Guide for pE-800 with pE-6501-8
USB-TTL Conversion kit
pE-Universal Collimator
Breakout cables
Liquid Light Guide
The CoolLED pE Driver is first required for software integration, and can be downloaded here.
Contact CoolLED for further information.
