CoolLED’s pE-300lite is a simple white light LED illumination system that offers intense, broad-spectrum LED illumination for fluorescence microscopy. The system is perfect for hospital and regular research laboratory environments and is affordable within consumables budgets.
This mercury-free alternative is safer, more controllable and precise than conventional bulb illumination options. Spectral coverage is from the UV (DAPI excitation) to the Red region (Cy5 excitation).
LED technology is quickly evolving, and since 2017 our new generation has double the irradiance at the sample plane, and for even higher irradiance is available as a Direct Fit model (which also reduces the cost to buy and run thanks to fewer consumables). With ACT Label certification, it’s even an environmentally friendly option for illumination.
The pE-300lite is a simple white light LED microscope illuminator. It provides a broad spectrum of illumination, covering the excitation bands of common fluorophores such as DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more. Operation is by a remote manual control pod with instant on/off and control of irradiance from 0-100%.
It is now possible to view and image your samples brighter than ever
As part of the award winning pE-300 Series, the enhanced pE-300lite now delivers double the irradiance at the microscope sample plane.
Convallaria sample captured using Photometrics Prime camera with 10 ms exposure.
With the new enhanced pE-300lite, this simple white light, at full power uses under 60 Watts. That is almost a quadruple efficiency increase, and compares to other LED technology which uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology, with no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.
With a long lifetime and a comprehensive range of microscope adaptors, the pE-300lite can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.
Direct fit or liquid light guide
There are two pE-300lite configuration options: direct fit or liquid light guide. The Direct Fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables. As a replacement to a metal halide source on a confocal microscope, the Liquid Light Guide model is particularly suitable for pre-aligning samples before scanning commences. In this way, photo bleaching of the sample can be minimised when LED illumination is used. More information on the available variants can be found under specifications.
Award winning technology
CoolLED’s pE-300 Series was the 2017 winner of the “Go Beyond Award” for “Excellence in sustainability in laboratory and other high technology facilities”. The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products, and projects that are advancing sustainable, high-performance facilities.
Simple to Buy
- Works with everyday fluorophores
- Affordable through lab consumables budget
- Broad spectrum – covers UV (DAPI) to Red region (Cy5)
- Specify for existing single and multi-band filter sets – no need to buy new filters
Simple to Fit
- Direct fit to the microscope in seconds
- Wide range of microscope adaptors – fits most microscopes
- No alignment, a once only adjustment
Simple to Use
- Instant on/off – no shutters required, no warm up or cool down
- Stable and repeatable – reliable and precise results
- Fine irradiance control in 1% steps (0-100%) – no ND filters required
- Excellent uniformity over field of view – fixed and stable, no alignment necessary
Plus all the benefits of LED technology
The system comprises a pE-300lite Light Source, power supply and control pod for operation and access to settings and usage history.
Manual control for instant on/off and irradiance control in 1% steps from 0 – 100%
There are several variants available, as explained below:
Choose direct fit or liquid light guide
There are two pE-300lite configuration options:
Direct Fit: Higher irradiance is possible when directly connecting to a microscope. Select from a range of microscope adaptors which covers all current and most older models. A simple once-only adjustment will allow optimisation to the optical path of the microscope. Specify from CoolLED’s range of epi-fluorescent or transmitted port microscope adaptors
Liquid Light Guide: If there is a need to keep the source of illumination remote from the microscope. This requires a fixed 3 mm diameter liquid light guide. An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required. We now include a light source stand for the pE-300lite Illumination Systems ordered for light guide delivery.
Choose single-band or multi-band
DAPI can be excited at either 365 nm or 400 nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted. To accommodate this, the pE-300lite can be specified in one of two waveband configurations to match existing filter sets within the microscope.
A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365 nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400 nm. The user can specify the configuration which is appropriate for their microscope.
100-240 V a.c. 50/60 Hz, 1.4 A
|pE-300 Series||Light Source||At the wall (includes power supply unit consumption)|
|Standby||2 W||3.3 W|
|Full white irradiance||46 W||56.4 W|
pE-300lite Light Source: 77 mm(w) x 186 mm(d) x 162(h) – Weight 1.4 kg
pE-300lite Control Pod: 88 mm(w) x 125 mm(d) x 37 mm(h) – Weight 0.32 kg
pE-300lite Power Supply: 167 mm(w) x 67 m(d) x 35 mm(h) – Weight 0.62 kg
pE-Universal Collimator: 44 mm(w) x 86 mm(d) x 44mm(h) – Weight 0.17 kg
Environment and Safety
- Mercury-free and laser-free
- Energy Efficient: 80% less power
- Long lifetime
- No bulb replacements
- Reduced risk of eye damage
- Quiet operation
- No special disposal regulations or issues
Industry-leading 36 months. Read more here.
After testing the CoolLED LED illumination systems, we decided to buy two CoolLED pE-300lite light sources to replace our old mercury excitation lamps. We use one of the light sources for a dissecting microscope, and the other for a 2-photon microscope. We are extremely satisfied with the products in that they are very bright and easy to use. The remote intensity adjustment is extremely convenient and the compact sizes are truly an advantage for environments with restricted space, like most laboratories. For these reasons, I strongly recommend the use of the CooLED pE-300lite LED illumination system.
Rodrigo Javier Gonzalez, Postdoctoral fellow, Department of Microbiology and Immunobiology, Harvard Medical School
Adopting LED light sources for fluorescence microscopy can help laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps.
Our CoolLED pE-300 Series is also ACT label certified, which stands for accountability, consistency and transparency. Introduced by My Green Lab, this provides an easy way for scientists to measure and compare the environmental impact of a product by reviewing the product itself, its manufacturing process, packaging and disposal. Visit our environment page to find out more.
|Light Source||Emission Colour||Type||CoolLED LED||Chroma||Semrock|
|pE-300lite SB, pE-300lite MB||Blue||Single||365 or 400||49053 CoolLED DAPI|
|pE-300lite SB||Blue||Single||365||39000 AT Dapi||49000 ET Dapi||LED-DAPI-B|
|pE-300lite SB||Cyan||Single||460||49001 ET eCFP||49013 ET Teal||LED-CFP-A||LED-mTFP-A|
|pE-300lite SB||Green||Single||460||49002 ET eGFP||39002 AT eGFP||GFP-4050B||GFP-A-Basic|
|pE-300lite SB||Green||Single||460||49054 CoolLED GFP|
|pE-300lite SB||Orange||Single||GYR (525-660)||49004 ET Cy3||49005 ET dsRed||TxRed-4040C||LED-TRITC-A|
|pE-300lite SB, pE-300lite MB||Red||Single||GYR (525-660)||49055 CoolLED mCherry #1||49055 CoolLED mCherry #2|
|pE-300lite SB||Red||Single||GYR (525-660)||49008 ET mCherry||39010 AT Tex. Red||YFP-2427B||LED-mCherry-A|
|pE-300lite SB||Far Red||Single||GYR (525-660)||49006 ET CY5||39007 AT Cy5||Cy5-4040C|
|pE-300lite MB||Blue||Single||400||49021 eBFP2||49028 ET Dapi 395||LED-DAPI-B|
|pE-300lite MB||Cyan||Single||460||49001 ET eCFP||49013 ET Teal||LED-CFP-A||LED-mTFP-A|
|pE-300lite MB||Green||Single||460||49054 CoolLED GFP|
|pE-300lite MB||Green||Single||460||49002 ET eGFP||39002 AT eGFP||GFP-4050B||GFP-A-Basic|
|pE-300lite MB||Orange||Single||GYR (525-660)||49004 ET Cy3||49005 ET dsRed||TxRed-4040C||LED-TRITC-A|
|pE-300lite MB||Red||Single||GYR (525-660)||49008 ET mCherry||39010 AT Tex. Red||YFP-2427B||LED-mCherry-A|
|pE-300lite MB||Far Red||Single||GYR (525-660)||49006 ET CY5||39007 AT Cy5||Cy5-4040C|
|pE-300-LT-D-SB-YYY-ZZ:||pE-300lite direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Single band filter set configuration|
|pE-300-LT-D-MB-YYY-ZZ:||pE-300lite direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Multi band filter set configuration|
|pE-300-LT-L-SB-ZZ:||pE-300lite Light Source for use with 3mm Liquid Light Guide. Single Band filter configuration. Includes light source, stand, control pod & power supply|
|pE-300-LT-L-MB-ZZ:||pE-300lite Light Source – Multi-Band. For use with 3mm Liquid Light Guide. Multi band filter configuration. Includes light source, stand, control pod & power supply|
|pE-1906||1.5 m long, 3 mm diameter liquid light guide|
|pE-10400-YYY||pE-Universal collimator for use with a single liquid light guide. Includes customer-specified adaptor|
To specify microscope code (YYY) see Adaptors
To specify power cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA
More information is available on the Accessories tab.
- Measuring illumination intensity with accuracy and precision
- Under the Microscope 2: LED microscopy illumination in an academic imaging facility
- Under the Microscope 1: LED illumination systems in the modern sustainable laboratory
- Optimal LED Filtering for Fluorescence Microscopy
- CoolLED pE-300 Series Leaflet
- CoolLED pE-300 Series Quick Start Guide
- CoolLED pE-300 Series User Manual
- CoolLED pE-300 Series Declaration of Conformity
- CoolLED pE-300 Series Troubleshooting Guide
- CoolLED pE-300lite Exploded Diagram
No imaging software availale for this product
The award winning pE-300 Series is a range of LED Illumination Systems for Fluorescence, Optogenetics, Electrophysiology and high speed microscopy applications. It comprises 3 systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra.
The table below is a quick reference guide to the features offered by the pE-300 Series systems.
|Simple to fit and use|
|No bulb replacement|
|Direct or Light Guide delivery options|
|Stable & Repeatable - 10-100% intensity control|
|Filter set compatibility|
|Individual channel intensity control|
|Individual channel selection|
|Integration with imaging software|
|Global remote trigginer (TTL, microsecond)|
|Individual channel triggering (TTL, microsecond)|
|Removable inline filter holders|