pE-300^lite

The pE-300^lite is a simple white light LED illuminator for microscopes. It provides a broad spectrum of illumination, covering the excitation bands of common fluorophores such as DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more. Operation is by a remote manual control pod with instant on/off and control of irradiance from 0-100%.

Direct fit or liquid light guide

There are two pE-300^lite configuration options: direct fit or liquid light guide. The direct fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables.

For confocal microscopes, the liquid light guide variant offers the ideal replacement to a metal halide light source, minimising photobleaching and phototoxicity when viewing samples before scanning. More information on the available variants can be found under specifications.

Energy efficiency for cost-saving sustainability

When it comes to making labs safer and more sustainable, LED microscope lighting is an attractive option when compared to old toxic mercury or metal halide lamps. All three channels of the pE-300^lite at full power use just 60 Watts, which is even lower than other LED technology which uses 120 to 350 Watts. “Green Grants” are even sometimes available from establishments, providing financial support owing to the reduction in institutional energy usage.

With a long lifetime and a comprehensive range of microscope adaptors, the pE-300^lite can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

Award winning technology

CoolLED’s pE-300 Series was the 2017 winner of the International Institute for Sustainable Laboratories (I2SL) “Go Beyond Award” for “Excellence in sustainability in laboratory and other high technology facilities”.

Simple to Buy

• Works with everyday fluorophores
• Affordable through lab consumables budget
• Broad spectrum – covers UV (DAPI) to Red region (Cy5)
• Specify for existing single and multi-band filter sets – no need to buy new filters

Simple to Fit

• Direct fit to the microscope in seconds
• Wide range of microscope adaptors – fits most microscopes
• No alignment, a once only adjustment

Simple to Use

• Instant on/off – no shutters required, no warm up or cool down
• Stable and repeatable – reliable and precise results
• Fine irradiance control in 1% steps (0-100%) – no ND filters required
• Excellent uniformity over field of view – fixed and stable, no alignment necessary

Plus all the benefits of LED technology

The system comprises a pE-300^lite Light Source, power supply and control pod for operation and access to settings and usage history.

Control

Manual:
Manual control for instant on/off and irradiance control in 1% steps from 0 – 100%

There are several variants available, as explained below:

Choosing configurations

There are two pE-300^lite configuration options:

Direct Fit: Higher irradiance is possible when directly connecting to a microscope. Select from a range of microscope adaptors which covers all current and most older models.  A simple once-only adjustment will allow optimisation to the optical path of the microscope. Specify from CoolLED’s range of epi-fluorescent or transmitted port microscope adaptors

Liquid Light Guide: If there is a need to keep the source of illumination remote from the microscope. This requires a fixed 3 mm diameter liquid light guide.  An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.  We now include a light source stand for the pE-300^lite Illumination Systems ordered for light guide delivery.

Choose single-band or multi-band

DAPI can be excited at either 365 nm or 400 nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted.  To accommodate this, the pE-300^lite can be specified in one of two waveband configurations to match existing filter sets within the microscope.

A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365 nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400 nm.  The user can specify the configuration which is appropriate for their microscope.

Specification

Power requirements
100-240 V a.c. 50/60 Hz

Power consumption

Dimensions

pE-300^lite Light Source: 77 mm(w) x 186 mm(d) x 162(h) – Weight 1.4 kg
pE-300^lite Control Pod: 88 mm(w) x 125 mm(d) x 37 mm(h) – Weight 0.32 kg
pE-300^lite Power Supply: 167 mm(w) x 67 m(d) x 35 mm(h) – Weight 0.62 kg

pE-Universal Collimator: 44 mm(w) x 86 mm(d) x 44mm(h) – Weight 0.17 kg

Environment and Safety

• Mercury-free and laser-free
• Energy Efficient
• Long lifetime
• No bulb replacements
• Reduced risk of eye damage
• Quiet operation
• No special disposal regulations or issues

Warranty

Industry-leading 36 months. Read more here. 

All data correct at time of publication

We are often asked about the power, intensity or irradiance of CoolLED Illumination Systems. The answer is not as simple as you might believe from many websites and data sheets, and it can be difficult to compare data from these sources as measuring set ups differ.

Photons are also lost as light travels from the light source to sample plane. The only way to objectively compare light sources is by measuring irradiance at the sample plane.  To understand why we use the term irradiance and how to measure and compare light sources with accuracy and precision, download our white paper. Or please contact us if you have further questions.

For performance data, please contact us

The scientific community is driving change across academia, industry and government to make laboratories more sustainable. When it comes to fluorescence microscopy illumination, switching to the pE-300 Series can help laboratories meet their sustainability targets, by consuming less energy and avoiding the use of toxic mercury.

Our CoolLED pE-300 Series is also ACT label certified, which stands for accountability, consistency and transparency. Introduced by My Green Lab, this provides an easy way for scientists to measure and compare the environmental impact of a product by reviewing the product itself, its manufacturing process, packaging and disposal. Visit our environment page to find out more.

“It’s great to see how My Green Labs and CoolLED are helping to move things ahead in the area of laboratory sustainability”. – Todd Lynch, UCLA Capital Programs

To specify microscope code (YYY) see Adaptors
To specify power cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

More information is available on the Accessories tab.