pE-10

Robust Data Generation 

While AI-assisted spectral unmixing is becoming a popular technique for overcoming the challenge of spectral overlap when imaging multiple fluorophores, results can be sensitive to user-defined parameters, raising questions around data accuracy and reproducibility.  

Combining the pE-10 Illumination System with carefully engineered Kromnigon SpectraSplit® 10  optical filters instead allows clean optical separation of up to 10 fluorophores during image acquisition itself. Avoiding the need for complex “black box” image manipulation algorithms significantly reduces ambiguity in the data, enabling reproducibility across time, instruments and laboratories – which is particularly valuable in regulated environments such as translational research and pathology.  

Faster Imaging, Higher Throughput  

Hardware-controlled illumination maximises system throughput and reduces time-to-result: 

• No spectral scanning overhead 

• Reduced validation and QC burden 

• <7 µs TTL for fast automation 

Choose Your Fluorophores  

Compatibility with a wide range of fluorophores allows flexibility to select cost-effective or high-performance fluorophores depending on the application, with confidence that the system can adapt as requirements evolve in the future.  

*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Aluora, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brilliant Violet dyes are trademarks of Sirigen/BD

Next generation high-plex imaging technology  

Supporting this advanced optical platform is the Kromnigon Streptaclick® labelling system, which delivers brighter signals, faster workflows and better results.  

For manufacturers of automated imaging systems, this combined approach provides straightforward access to next-generation high-plex imaging technology, reducing developmental risk and accelerating time-to-market in the increasingly competitive spatial biology arena.    

• Feature	CoolLED pE-10 & Kromnigon SS10 package	CoolLED pE-10
  Simple access to validated optical platform for next generation high-plex imaging	X
  Hardware-based spectral separation for robust, reproducible data	X
  System-validated list of compatible fluorophores	X
  10 individually controllable LEDs (365 – 850 nm)	X	X
  0-100% irradiance control per channel to balance fluorophore brightness	X	X
  Highly repeatable <7 µs TTL triggering	X	X
  Homogenous illumination across multiplex channels, ideal for quantification and slide scanning	X	X
  Can be used as a stand-alone LED Illumination System		X
  Custom 10-channel Amora available for bespoke requirements

Control & Interface

USB: USB 2.0 for PC connection; supports software and LightBridge GUI operation. USB-C interface.

TTL: 10 TTL inputs allow independent on/off control of each channel via 15 Way D-Type connector interface. Global TTL for on/off synchronisation to camera via BNC interface. Triggering speed <7 µs.

Analogue: 10 analogue inputs, 0–10 V, 0–50 kHz, for dynamic control of irradiance from external analogue signals via 15 Way D-Type connector interface.

RS-232: Standard serial interface for external control and automation via 15 Way D-Type connector interface.

Ethernet: Ethernet interface for networked control where required.

Graphical user interface (GUI): CoolLED LightBridge GUI operates via USB interface to allow on/off control, LED selection, real-time irradiance control, Sequence Runner, saving and loading pre-sets, pE-10 start-up settings, and analogue settings.

Sequence Runner: Combining software control with the speed of TTL, the Sequence Runner program allows LED sequences and irradiance to be programmed via LightBridge. The sequences are controlled via global TTL from a single TTL input, such as from a camera TTL out or other external hardware, and therefore require minimal electronic hardware.

Light Delivery: Light delivery via the standard 3 mm liquid light guide. The pE-Universal Collimator and specified microscope adaptor are also available.

Power

Power requirements: 100–240 V a.c., 50/60 Hz.

Power consumption:
Maximum (10 LEDs at 100% irradiance): 125 W
Standby: 6 W

Dimensions

pE-10 Light Source: 160 mm (w) x 180 mm (d) x 145 mm (h) — Weight 3 kg

pE-10 Power Supply: 164 mm (w) x 64 mm (d) x 35 mm (h) — Weight 0.58 kg

pE-Universal Collimator: 44 mm (w) x 86 mm (d) x 44 mm (h) — Weight 0.17 kg

We are often asked about the power, intensity or irradiance of CoolLED Illumination Systems. The answer is not as simple as you might believe from many websites and data sheets, and it can be difficult to compare data from these sources as measuring set ups differ.

Photons are also lost as light travels from the light source to sample plane. The only way to objectively compare light sources is by measuring irradiance at the sample plane.  To understand why we use the term irradiance and how to measure and compare light sources with accuracy and precision, download our white paper. Or please contact us if you have further questions.

For performance data, please contact us

CoolLED designs and manufactures LED illumination for microscopy and is ideally placed to deliver customised OEM solutions.  

The pE-10 is available in the Amora Series: our proven off-the-shelf product range adapted to offer unparalleled customisation opportunities. 

• Streamlined development using proven off-the-shelf technology 

• Professional expert support 

• Market-leading warranty 

• ‘Try before you buy’ option 

• Trusted experts in LED illumination technology since 2006 

Click the button to find out more. 

LED products help laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps. CoolLED’s products have the following benefits: 

• Mercury-free and laser-free 

• Energy Efficient 

• Long lifetime 

• No bulb replacements 

• Reduced risk of eye damage 

• Quiet operation 

• No special disposal regulations or issues 

Optimised for use with Kromnigon SpectraSplit 10 optical filter set. Visit the Kromnigon web page for more information.   

To specify microscope adaptor code (YYY), see Adaptors (www.coolled.com/products/adaptors) 

To specify local power cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA