Welcome to CoolLED

The pE-300^white has been a great asset for a core facility. It has meant less time spent changing bulbs (so less downtime to the user and more productive time for ourselves) and no longer worrying about users turning the light source on and off throughout the day as multiple users come and go.  The controllable intensity has also proved highly popular with users who are now able to set the minimum light level required to scan their samples and minimise the risk of phototoxic damage and bleaching to their specimens

We chose the CoolLED light source with our live cell imaging system because we need a fluorescence light source that is efficient, easy to use and has a very bright and robust signal for fluorescence – but is also incredibly gentle on cell samples. Our system is designed around a lack of phototoxicity, so when using fluorescence with holotomography, we really need the ideal excitation that keeps cells happy, but shows exactly what is labelled.

We needed an easy to install, triggerable, intensity-modulated light source. An LED is by far the easiest and cheapest solution. Furthermore, small latencies between triggered light pulses are crucial for the analysis of the kinetics of measurements. We used the CoolLED pE-300^ultra, which meets these requirements. Another great advantage of this product lies in its versatility for use in upcoming experiments covering different wavelengths. Its flexibility allows the performance of very complex experimental protocols.

All our lab members have been very glad that the microscope representative recommended CoolLED pE-300^white to us. It is really cool! With this new and award-winning technology, we got rid of the big and heavy fluorescence power supply box and camera controller, and will never worry about the life time of a fluorescence lamp again, because we can use the pE-300^white almost forever. Now we can turn the fluorescence on or off instantly and adjust the intensity precisely and conveniently with no shutters required. In addition, the illumination is clean, bright and stable across the spectrum, and it is not just safer for us but also energy-efficient for our environment. We love it! Thank you, CoolLED!

We are extremely happy with our CoolLED pE-300^white System. Integration with our microscope was simple, and the robust system has never needed maintenance or alignment. I am particularly happy with the user interface as it is simple and intuitive.

I have been using a pE-4000 for optogenetic experiments using in vitro slices for just over a year now. The pE-4000 has been fantastic for my needs: the ability to simultaneously use multiple wavelengths of light has allowed me to carry out experiments using two different opsins to study how external inputs converge on hippocampal interneurons. I’m currently setting up my own research group at the University of Exeter, and all of my patch-clamp rigs will have a pE-4000 on them, as they are such versatile devices for optogenetic experiments.