The pE-100 series is a family of single-wavelength fluorescence LED illumination systems, which are compact and simple to use. Systems can be specified at any one of 20 different LED wavelengths, and are ideal for routine clinical screening, electrophysiology or basic research applications. Operation is by a remote manual control pod with instant on/off and irradiance control from 0-100%, with remote control available via a TTL trigger.
The pE-100 system is designed for fluorescence applications requiring a single LED excitation wavelength. It is perfect for clinical applications such as routine screening (e.g. using Auramine for Tuberculosis), for research applications requiring precise irradiance control and fast-switching, or for electrophysiology applications where light has to be delivered to a specific location. The user can select from 20 different LED wavelengths, ranging from the near-UV at 365 nm to the near-IR at 770 nm. For increased irradiance, a direct fit version is also available, which fits directly to the microscope via a specified adaptor.
- Broadest LED spectrum available: 365-770 nm: replaces metal halide or mercury
- Compatible with existing single and multi-band filter sets: no need to buy new filters
- Simple to fit, simple to use: no alignment, a once only adjustment
- Stable and repeatable: reliable and consistent results
- Wide range of microscope adaptors: fits most microscopes
- Precise irradiance control in 1% steps (0-100%): no ND filters required
- Excellent uniformity over field of view: fixed and stable, no alignment necessary
The system comprises a pE-100 LED Light Source, control pod, and power supply.
Control pod for operation and access to settings and usage history.
Manual control for instant on/off and irradiance control in 1% steps from 0 – 100%
Via single TTL for on/off control using a BNC connection on the control pod. Triggering speed approx. 150 µs
There are three standard pE-100 configuration options:
- Direct-fit: The pE-100 is designed to connect directly to a microscope’s epi-illumination port by selecting from a range of microscope adaptors. A simple once only adjustment will allow optimisation to the optical path of the microscope (watch the tutorial video here).
- Liquid Light Guide: The pE-100light guide connects to the microscope via a fixed 3 mm diameter, 1.5 m long liquid light guide.
- Fibre: The pE-100fibre features an SMA connection for accepting multimode fibres and has been designed with efficient coupling into a wide range of fibre types. The pE-100fibre also includes excitation filter holders.
Combining pE-100 Light Sources
- Direct fit configuration
pE-100 units can be combined using the pE-Combiner for applications requiring a second LED wavelength with independent control and triggering.
100-240 V a.c. 50/60 Hz
2.2 W (standby) 25 W (full irradiance)
pE-100 Light Source (direct fit): 60 mm(w) x 170 mm(d) x 70 mm(h) – Weight 0.38 kg
pE-100light guide Light Source: 52 mm(w) x 95 mm(d) x 52 mm(h) – Weight 0.42 kg
pE-100fibre Light Source: 76 mm(w) x 165 mm(d) x 80 mm(h) – Weight 0.42 kg
Combined 2 x pE-100fibre: 190 mm(w) x 220 mm(d) x 190 mm (h) – Weight 1.5 kg
pE-100 Control Pod: 102 mm(w) x 110 mm(d) x 50 mm(h) – Weight 0.55 kg
pE-100 Power Supply: 55 mm(w) x 95 mm(d) x 40 mm(h) – Weight 0.19 kg
Environment and Safety
- Mercury-free and Laser-free
- Energy Efficient
- Long lifetime
- No bulb replacements
- Reduced risk of eye damage
- Quiet operation
- No special disposal regulations or issues
Industry-leading 36 months. Read more here.
All data correct at time of publication
We are often asked about the power, intensity or irradiance of CoolLED Illumination Systems. The answer is not as simple as you might believe from many websites and data sheets, and it can be difficult to compare data from these sources as measuring set ups differ.
Photons are also lost as light travels from the light source to sample plane. The only way to objectively compare light sources is by measuring irradiance at the sample plane. To understand why we use the term irradiance and how to measure and compare light sources with accuracy and precision, download our white paper. Or please contact us if you have further questions.
For performance data, please contact us
We regularly perform assays which look at both the host and pathogen, where keeping both alive and happy is no mean feat. Luckily, we can employ low-power, fast-trigger excitation LEDs with our pE-Series Illumination Systems to perform live cell imaging, involving calcium signalling, fluorescent anti-microbial peptide dynamics and the fungal/host cell biology therein at high temporal frame rates.
Dr Darren Thomson, Experimental Officer at the Manchester Fungal Infection Group, UoM (pE-100)
CoolLED pE-100 (470 nm) provides very bright and stable excitation light for GCaMP3 imaging. Use of LEDs is very easy and convenient, additionally TTL trigger on and off provides some flexibility.
Chuanqiang Zhang, PhD student, Hans-Berger Department of Neurology, University Hospital Jena (pE-100)
I originally approached various companies with regards to getting an LED light source for my Leica DMLB microscope. The fact that the microscope was old and had a mechanised stage meant that some of the LED systems that we looked at were unsuitable to be fitted. Thankfully the CoolLED system simply slots in where the old mercury bulb unit used to sit. It took less than five minutes to fit, and was ready to use once it was switched on. No more waiting for the bulb to get to temperature, no more bulb changing, or re-calibrating of the mirrors before use. Simply marvellous!
Kam Yim Wong, Immunology Dept, Derriford Hospital, Plymouth UK (pE-100)
We have had great results using the CoolLED pE-100 on our TB, fungus and bacteria screening microscopes and find the CoolLED LED products make screening quick and easy.
Richard Wills, Microbiology Laboratory Manager at The Royal Berkshire Hospital (pE-100)
The main benefit of CoolLED's light source is the ability to switch on and off when required and also not having to align or change a bulb every 300 hours. Over the years it will pay for itself when compared to the cost of mercury bulbs.
Kevin MacKenzie, Histology/EM and Imaging Facility, Institute of Medical Sciences, Aberdeen University (pE-100)
- Target-specific control of olfactory bulb periglomerular cells by GABAergic and cholinergic basal forebrain inputs
- Skeletal muscle derived Musclin protects the heart during pathological overload
- Reprogramming reactive glia into interneurons reduces chronic seizure activity in a mouse model of mesial temporal lobe epilepsy
- The paraventricular thalamus provides a polysynaptic brake on limbic CRF neurons to sex-dependently blunt binge alcohol drinking and avoidance behavior in mice
- Loss of Tsc1 from striatal direct pathway neurons impairs endocannabinoid-LTD and enhances motor routine learning
|244-87-XXX-YYY-ZZ:||pE-100 direct fit Light Source, fixed collimating adaptor to customer-specified microscope, remote manual control pod, and power supply.|
|244-87-XXX-L15-ZZ:||pE-100light guide Light Source with fixed 3 mm diameter, 1.5 m long liquid light guide, remote manual control pod, and power supply.|
|pE-100-F-XXX-ZZ:||pE-100fibre Light Source, remote manual control pod, SMA fibre connection, and power supply (no fibre provided).|
|pE-100-F-XXX-XXX-ZZ:||Combination of two pE-100fibre Light Sources, remote manual control pod, SMA fibre connection, and power supply. Accepts 25 mm diameter excitation filters (fibre and filters not provided).|
- Wavelength (XXX) see Description tab above for wavelength Spectrum.
- Microscope code (YYY) from Adaptors
- Power cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA
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