Overview

CoolLED’s pE-300white offers intense, broad-spectrum LED illumination for fluorescence microscopy that is perfect for hospital and regular research laboratory environments. The system is a mercury-free alternative that is safer, more controllable and precise than traditional bulb illumination options.  Spectral coverage is from the UV (DAPI excitation) to the red region (Cy5 excitation).

LED technology is quickly evolving, and since 2017 our new generation has double the intensity at the sample plane, and for even higher intensity is available as a Direct Fit model (which also reduces the cost to buy and run thanks to fewer consumables). With ACT Label certification, it’s even an environmentally friendly option for illumination.

The pE-300white is a compact and simple to use LED illumination unit.  It provides a broad spectrum of illumination, covering the excitation bands of common fluorophores such as DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.  Operation is by a manual control pod with instant on/off and intensity control from 0-100%, with remote control available via USB or TTL trigger.

It is now possible to view and image your samples brighter than ever

As part of the award winning pE-300 Series, the enhanced pE-300white now delivers double the intensity at the microscope sample plane.

1907005-pE-300 Series images

Convallaria sample captured using Photometrics Prime camera with 10 ms exposure.

With the new enhanced pE-300white, all three channels at full power use just 60 Watts. That is almost a quadruple efficiency increase. This compares to other LED technology which uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology.  Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.

Fast multi-channel imaging without pixel shift

An additional benefit of the pE-300white is that fluorophores can be viewed either individually or in combination, without filter cube changes.  This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used.  Independent control of the three LED wavebands allows control of the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.

Direct fit or liquid light guide

There are two pE-300white configuration options: direct fit or liquid light guide. The Direct Fit variant delivers higher intensity and reduces the cost to buy and run thanks to fewer consumables. As a replacement to a metal halide source on a confocal microscope, the Liquid Light Guide model is particularly suitable for pre-aligning samples before scanning commences.  In this way, photo bleaching of the sample can be minimised when LED illumination is used. More information on the available variants can be found under specifications .

Award winning technology

CoolLED’s pE-300 Series was the 2017 winner of the “Go Beyond Award” for “Excellence in sustainability in laboratory and other high technology facilities”. The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products, and projects that are advancing sustainable, high-performance facilities.

Performance
  • Remote control via USB or TTL trigger
  • Clean bright illumination across the spectrum: excites common fluorophores
  • Three LEDs
  • Instant on/off: No shutters required, no warm up or cool down
  • Stable and repeatable: reliable and consistent results
  • Precise intensity control in 1% steps (0-100%): no ND filters required
Convenience
  • Specify for existing single and multi-band filter sets: no need to buy new filters
  • Simple to fit, simple to use: no alignment, a once only adjustment
  • Wide range of microscope adaptors: fits most microscopes
Plus all the benefits of LED technology

The system comprises a pE-300white Light Source, control pod, and power supply.

Control

Control pod for operation and access to settings and usage history.

Manual:
Manual control for instant on/off and intensity control in 1% steps from 0 – 100%
Individual bands can be selected/deselected and controlled independently as desired

Remote:
Via single TTL for on/off control of selected channels using a BNC connection on the light source.
Triggering speed <20 µs

Connectivity:
Remote via USB (B type) for imaging software control (see Imaging Software)

 

There are several variants available, as explained below:

Choose direct fit or liquid light guide

There are two pE-300white configuration options:

Direct Fit: Higher intensity is possible when directly connecting to a microscope. Select from a range of microscope adaptors which covers all current and most older models.  A simple once-only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide: If there is a need to keep the source of illumination remote from the microscope. As a replacement to a metal halide source on a confocal microscope, the liquid light guide configuration is particularly suitable for pre-aligning samples before scanning commences.  In this way, photo bleaching of the sample can be minimised when LED illumination is used. This requires a fixed 3 mm diameter liquid light guide.  An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.  We now include a light source stand for the pE-300white Illumination Systems ordered for light guide delivery.

Choose single band or multi band variant

DAPI can be excited at either 365 nm or 400 nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted.  To accommodate this, the pE-300white can be specified in one of two waveband configurations to match existing filter sets within the microscope.

A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365 nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400 nm.  The user can specify the configuration which is appropriate for their microscope.

Specification

Power requirements
100-240 V a.c. 50/60 Hz, 2 A

Power consumption

pE-300 Series Light Source At the wall (includes power supply unit consumption)
Standby 2 W 3.3 W
1 band (GYR) 20 W 24.4 W
2 bands (BLU & GYR) 38 W 44.3 W
Full white intensity (3 bands) 46 W 56.4 W

 

Dimensions
pE-300white Light Source: 77 mm(w) x 186 mm(d) x 162 mm(h) – Weight 1.4 kg
pE-300white Control Pod: 88 mm(w) x 125mm(d) x 37 mm(h) – Weight 0.32 kg
pE-300white Power Supply: 167 mm(w) x 67 m(d) x 35 mm(h) – Weight 0.62 kg

pE-Universal Collimator: 44 mm(w) x 86 mm(d) x 44 mm(h) – Weight 0.17 kg

Environment & Safety

  • Mercury-free & Laser-free
  • Energy Efficient: 80% less power
  • Long lifetime
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues

Warranty
System: 36 months

  • We find the system to be excellent, it gives us bright fluorescence and being able to switch the light on and off without having to worry about warming up/cooling down is a big advantage over our old system.

    Dr Kat Millen , Lab Manager , University of Cambridge, United Kingdom

  • We’ve just bought the CoolLED pE-300white, the little brother to the pE-4000 (on the confocal, we don’t need the more powerful version), it’s really nice.

    Dr Martin Spitaler, Head of Imaging Facility, Max Planck Institute of Biochemistry, Germany

  • We are extremely happy with our CoolLED pE-300white System. Integration with our microscope was simple, and the robust system has never needed maintenance or alignment. I am particularly happy with the user interface as it is simple and intuitive.

    David Inglis, Senior Lecturer, Dept of Engineering, Macquarie University, Australia

  • I just signed off on the installation of our new CoolLED pE-300white,  fitted today on our Leica SP5 confocal. Happy to see the Hg bulb made obsolete! I love it and I’m sure the users will too.

    Rosey van Driel, Manager for Transmission Electron Microscopy (TEM), Institute for Frontier Materials, Deakin University, Australia

  • The pE-300white has been a great asset for a core facility. It has meant less time spent changing bulbs (so less downtime to the user and more productive time for ourselves) and no longer worrying about users turning the light source on and off throughout the day as multiple users come and go.  The controllable intensity has also proved highly popular with users who are now able to set the minimum light level required to scan their samples and minimise the risk of phototoxic damage and bleaching to their specimens.

    Peter O'Toole, Head of Imaging & Cytometry - University of York

  • Our imaging facility houses a range of different confocal microscopes from major vendors such as Olympus, Zeiss and Nikon.  All of these systems, including one purchased last year, were supplied with either a mercury arc burner or metal halide light source, used for observation of samples by eye prior to imaging using the confocal laser scanning system.  These light sources suffer many shortcomings, such as the requirement to frequently replace expensive components (burners, light guides), the need for heating/cooling cycles when turning on and off, safety issues regarding risk of explosion and problems with the disposal of mercury-containing bulbs.  For these reasons we decided to replace all of our confocal epi-illuminators with the CoolLED pE-300white.  Users now have the benefit of immediate on/off with no requirement for warm-up/cool-down cycles, they can fine-tune the intensity of illumination to match their labelling, there is no risk of explosion and there are no problems with the disposal of mercury-containing components.  We have found the CoolLED pE-300white to be highly reliable, simple to operate and work very well with all the standard fluorophores we use including DAPI, Alexa fluor 488, Alexa fluor 568.  To my knowledge there have been no instances where the LED light source has been judged inferior to the illuminator it replaced, with most users appreciating the benefits.  As a facility, the decision to replace our epi-illuminators with LEDs was seen as an investment that should be recovered within three years, with the projected lifetime of the LED illuminators considerably exceeding this time.

    Simon Walker, Imaging Facility Manager - Babraham Institute

  • The CoolLED pE-300white is the perfect fluorescence illumination system for teaching bio-medical students.  It is not only bright enough, but the ability to simultaneously illuminate 3 fluorescent proteins means students can observe structures with better context therefore achieving a more efficient learning outcome.

    Urs Ziegler, Ph.D. Head of Center for Microscopy and Image Analysis, University of Zurich

  • The lamp is on the microscope and is really impressive. A great product!

    Viki Allan, Professor of Cell Biology, University of Manchester

  • The CoolLED pE-300white is a perfect fluorescence illumination system for fixed fluorescence samples. We used it on a Nikon E400 upright microscope with four fluorescence channels. With a triple-band filter, it can also be used for simultaneous illumination of three fluorescent proteins. As a system running in a microscope facility, it provides excellent flexibility, enough brightness in all bands to most samples, and is cost-effective, stable, trouble-free, and easy to operate

    Dr. Yan Gu, Manager, Analytical and Quantitative Light Microscopy Facility, Genome Damage and Stability Centre, University of Sussex

  • We are absolutely delighted with the pE-300white light source - it is fabulous. We use it as illumination on our Zeiss Axiskop to basically just identify the objects of desire for further analysis with laser scan of our confocal system. The CoolLED pE-300 has replaced our old and worn out mercury lamp - we love it for its simplicity in use, and even more for its really bright illumination. Before we had to guess the objects, now we can see them clearly. What a difference!

    Prof. Dr. A. Preiss, University of Hohenheim

  • Our customer required a fluorescence light source for their new microscope and had enquired about a metal halide light source unaware of the CoolLED pE-300 Series. Once we had demonstrated the pE-300white they were immediately impressed with its capabilities. The ability to control the intensity of the light and the fact that it does not involve changing bulbs were major attractions. Through their work with mosquitos they hope to reduce the spread of diseases such as malaria. To do this they need to be able to clearly see which mosquitoes are fluorescing and the CoolLED pE-300white provides perfect excitation.

    Alan Todd, Microscope Services  Ltd, UK

CoolLED Optimised filters

CoolLED Optimised filters

Cell Viability, Image Quality & Sustainability

Cell Viability, Image Quality & Sustainability

Using Optogenetics to Analyse Neuronal Circuits

Using Optogenetics to Analyse Neuronal Circuits

Adopting LED light sources for fluorescence microscopy can help laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps.

Our CoolLED pE-300 Series is also ACT label certified, which stands for accountability, consistency and transparency. Introduced by My Green Lab, this provides an easy way for scientists to measure and compare the environmental impact of a product by reviewing the product itself, its manufacturing process, packaging and disposal. Visit our environment page to find out more.

Light SourceEmission ColourTypeCoolLED LEDChromaSemrock
pE-300white SB, pE-300white MBBlueSingle365 or 40049053 CoolLED DAPI
pE-300white SBBlueSingle36539000 AT Dapi49000 ET DapiLED-DAPI-B
pE-300white SBCyanSingle46049001 ET eCFP49013 ET TealLED-CFP-ALED-mTFP-A
pE-300white SBGreenSingle46049002 ET eGFP39002 AT eGFPGFP-4050BGFP-A-Basic
pE-300white SBGreenSingle46049054 CoolLED GFP
pE-300white SBOrangeSingleGYR (525-660)49004 ET Cy349005 ET dsRedTxRed-4040CLED-TRITC-A
pE-300white SB, pE-300white MBRedSingleGYR (525-660)49055 CoolLED mCherry #149055 CoolLED mCherry #2
pE-300white SBRedSingleGYR (525-660)49008 ET mCherry39010 AT Tex. RedYFP-2427BLED-mCherry-A
pE-300white SBFar RedSingleGYR (525-660)49006 ET CY539007 AT Cy5Cy5-4040C
pE-300white MBBlueSingle40049021 eBFP249028 ET Dapi 395LED-DAPI-B
pE-300white MBCyanSingle46049001 ET eCFP49013 ET TealLED-CFP-ALED-mTFP-A
pE-300white MBGreenSingle46049054 CoolLED GFP
pE-300white MBGreenSingle46049002 ET eGFP39002 AT eGFPGFP-4050BGFP-A-Basic
pE-300white MBOrangeSingleGYR (525-660)49004 ET Cy349005 ET dsRedTxRed-4040CLED-TRITC-A
pE-300white MBRedSingleGYR (525-660)49008 ET mCherry39010 AT Tex. RedYFP-2427BLED-mCherry-A
pE-300white MBFar RedSingleGYR (525-660)49006 ET CY539007 AT Cy5Cy5-4040C
pE-300white MBBlue-Green-RedMultiband400, 460, GYR (525-660)69401 – ET Triple Multi LEDLED-DA/FI/TX-A
pE-300white SBBlue-Green-RedMultiband365, 460, GYR (525-660)69401 – ET Triple Multi LEDLED-DA/FI/TX-A
pE-300white SBGreen-RedMultiband460, GYR (525-660)GFP/DsRed-A
pE-300white SBGreen-RedMultiband460, GYR (525-660)59022 ET eGFP/mCherryFITC/TxRed-A
pE-300white MBBlue-GreenMultiband400, 46059001v2 ET DAPI/Green FISH
pE-300white MBBlue-OrangeMultiband400, GYR (525-660)59002v2 ET DAPI/Orange
pE-300white MBBlue-Green-RedPinkel400, 460, GYR (525-660)89084v2 ET DAPI/Green/Orange FISHLED-DA/FI/TX-3X-B
pE-300white MBGreen-RedPinkel460, GYR (525-660)59222 ET eGFP/mCherryGFP/DsRed-2X-A
pE-300white MBBlue-RedMultiband400, GYR (525-660)59003 v2 ET DAPI/Red FISH
pE-300white SBBlue-Green-RedPinkel365, 460, GYR (525-660)LED-DA/FI/TX-3X-B
pE-300white SBGreen-RedPinkel460, GYR (525-660)59022 ET eGFP/mCherryGFP/DsRed-2X-A
pE-300white MBGreen-RedMultiband460, GYR (525-660)GFP/DsRed-A
pE-300white MBGreen-RedMultiband460, GYR (525-660)59022 ET eGFP/mCherryFITC/TxRed-A
pE-300-W-D-SB-YYY-ZZ: pE-300white direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Single band filter set configuration
pE-300-W-D-MB-YYY-ZZ: pE-300white direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Multi band filter set configuration
pE-300-W-L-SB-ZZ: pE-300white Light Source for use with 3 mm Liquid Light Guide.  Single Band filter configuration.  Includes light source, stand, control pod & power supply
pE-300-W-L-MB-ZZ: pE-300white Light Source – Multi-Band.  For use with 3 mm Liquid Light Guide.  Multi band filter configuration.  Includes light source, stand, control pod & power supply
pE-1906 1.5 m long, 3 mm diameter liquid light guide
pE-10400-YYY pE-Universal collimator for use with a single liquid light guide.  Includes customer-specified adaptor

To specify microscope code (YYY) see Adaptors
To specify power cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

More information is available on the Accessories tab.

pE-Universal Collimator

pE-Universal Collimator

Liquid Light Guide

Liquid Light Guide

pE-6501

pE-6501

Adaptors

Adaptors

The CoolLED pE Driver is first required for software integration, and can be downloaded here.

CompanySoftwareSummaryScreenshotQuick Start Guide
AndorAndor iQ
HamamatsuHCImage
Intelligent Imaging Innovations (3i)Slidebook
LaVision BioTecImSpector Pro 
LeicaLASX
MicromanagerMicromanager
Molecular DevicesMetaMorph
NikonNIS Elements
OlympusCellSens
Visitron SystemsVisiview 
ZeissZEN 

CoolLED Proprietary ZEN Bridge software is available – Contact CoolLED for further information.

The award winning pE-300 Series is a range of LED Illumination Systems for Fluorescence, Optogenetics, Electrophysiology and high speed microscopy applications. It comprises 3 systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra.

The table below is a quick reference guide to the features offered by the pE-300 Series systems.

Features pE-300lite pE-300white pE-300ultra
Broad spectrum
Simple to fit and use
Instant on/off
No bulb replacement
Direct or Light Guide delivery options
Long lifetime
Stable & Repeatable - 10-100% intensity control
Filter set compatibility
Mercury free
Individual channel intensity control
Individual channel selection
Integration with imaging software
Global remote trigginer (TTL, microsecond)
Individual channel triggering (TTL, microsecond)
Removable inline filter holders
Sequence Runner