pE-300^white

• We were looking for a cost efficient light source for epi-fluorescence for basic cell biology application. We bought the CoolLED pE-300^white. It is not only a very cost efficient solution compared to other LED-based illuminators but also a superior performance. The next light source will be for sure from CoolLED.

  Dr Tim Kaminski, In Singulo Solutions AB

• We have been using the pE-300^white  for several months now. This unit is amazing, and our technicians love the ease of use. From installing it to the ability to isolate light channels with a push of a button, has made out lives easier. The unit takes up less space than mercury burners, you don’t have to worry about the burner lifespan, and the heat produced by mercury burners. This unit is compact and the control box has a small footprint to maximise space around work area. If you haven’t tried this light source, you need to.

  Ken Reese, VP Laboratory Management, BrainBits, LLC

• All our lab members have been very glad that the microscope representative recommended CoolLED pE-300^white to us. It is really cool! With this new and award-winning technology, we got rid of the big and heavy fluorescence power supply box and camera controller, and will never worry about the life time of a fluorescence lamp again, because we can use the pE-300^white almost forever. Now we can turn the fluorescence on or off instantly and adjust the intensity precisely and conveniently with no shutters required. In addition, the illumination is clean, bright and stable across the spectrum, and it is not just safer for us but also energy-efficient for our environment. We love it! Thank you, CoolLED!

  Dr Tian Luo, Research Scientist, University of Texas Medical Branch

• CoolLED Illumination Systems are fantastic, and I really want to highlight how easy they are to use, which is great from a training perspective. In terms of microscopy, the flexibility of selecting and tuning individual channels is beneficial to prevent photobleaching.  LED microscope lighting has improved so much in the last few years, and every layer of extra capability makes the microscope setup more interesting to use.

  Alfonso Schmidt, Bioimaging Specialist, Hugh Green Cytometry Centre, Malaghan Institute of Medical Research

• The introduction of CoolLED Illumination Systems into the laboratories where I have worked has improved the use of standard light microscopy and also fluorescence microscopy. Standardised light sources to the specific wavelengths required provides the technician with the confidence to be able to provide high quality and reproducible results.

  Collette Allen, Salisbury NHS Trust, United Kingdom

• The unit is impressive compared to the previous mercury bulb set up we’ve been using.

  Simon Marshall, Laboratory Manager Carmarthen Veterinary Investigation Centre

• We find the system to be excellent, it gives us bright fluorescence and being able to switch the light on and off without having to worry about warming up/cooling down is a big advantage over our old system.

  Dr Kat Millen , Lab Manager , University of Cambridge, United Kingdom

• We’ve just bought the CoolLED pE-300^white, the little brother to the pE-4000 (on the confocal, we don’t need the more powerful version), it’s really nice.

  Dr Martin Spitaler, Head of Imaging Facility, Max Planck Institute of Biochemistry, Germany

• We are extremely happy with our CoolLED pE-300^white System. Integration with our microscope was simple, and the robust system has never needed maintenance or alignment. I am particularly happy with the user interface as it is simple and intuitive.

  David Inglis, Senior Lecturer, Dept of Engineering, Macquarie University, Australia

• I just signed off on the installation of our new CoolLED pE-300^white,  fitted today on our Leica SP5 confocal. Happy to see the Hg bulb made obsolete! I love it and I’m sure the users will too.

  Rosey van Driel, Manager for Transmission Electron Microscopy (TEM), Institute for Frontier Materials, Deakin University, Australia

• The pE-300^white has been a great asset for a core facility. It has meant less time spent changing bulbs (so less downtime to the user and more productive time for ourselves) and no longer worrying about users turning the light source on and off throughout the day as multiple users come and go.  The controllable intensity has also proved highly popular with users who are now able to set the minimum light level required to scan their samples and minimise the risk of phototoxic damage and bleaching to their specimens.

  Peter O'Toole, Head of Imaging & Cytometry - University of York

• Our imaging facility houses a range of different confocal microscopes from major vendors such as Olympus, Zeiss and Nikon.  All of these systems, including one purchased last year, were supplied with either a mercury arc burner or metal halide light source, used for observation of samples by eye prior to imaging using the confocal laser scanning system.  These light sources suffer many shortcomings, such as the requirement to frequently replace expensive components (burners, light guides), the need for heating/cooling cycles when turning on and off, safety issues regarding risk of explosion and problems with the disposal of mercury-containing bulbs.  For these reasons we decided to replace all of our confocal epi-illuminators with the CoolLED pE-300^white.  Users now have the benefit of immediate on/off with no requirement for warm-up/cool-down cycles, they can fine-tune the intensity of illumination to match their labelling, there is no risk of explosion and there are no problems with the disposal of mercury-containing components.  We have found the CoolLED pE-300^white to be highly reliable, simple to operate and work very well with all the standard fluorophores we use including DAPI, Alexa fluor 488, Alexa fluor 568.  To my knowledge there have been no instances where the LED light source has been judged inferior to the illuminator it replaced, with most users appreciating the benefits.  As a facility, the decision to replace our epi-illuminators with LEDs was seen as an investment that should be recovered within three years, with the projected lifetime of the LED illuminators considerably exceeding this time.

  Simon Walker, Imaging Facility Manager - Babraham Institute

• The CoolLED pE-300^white is the perfect fluorescence illumination system for teaching bio-medical students.  It is not only bright enough, but the ability to simultaneously illuminate 3 fluorescent proteins means students can observe structures with better context therefore achieving a more efficient learning outcome.

  Urs Ziegler, Ph.D. Head of Center for Microscopy and Image Analysis, University of Zurich

• The lamp is on the microscope and is really impressive. A great product!

  Viki Allan, Professor of Cell Biology, University of Manchester

• The CoolLED pE-300^white is a perfect fluorescence illumination system for fixed fluorescence samples. We used it on a Nikon E400 upright microscope with four fluorescence channels. With a triple-band filter, it can also be used for simultaneous illumination of three fluorescent proteins. As a system running in a microscope facility, it provides excellent flexibility, enough brightness in all bands to most samples, and is cost-effective, stable, trouble-free, and easy to operate

  Dr. Yan Gu, Manager, Analytical and Quantitative Light Microscopy Facility, Genome Damage and Stability Centre, University of Sussex

• We are absolutely delighted with the pE-300^white light source - it is fabulous. We use it as illumination on our Zeiss Axioskop to basically just identify the objects of desire for further analysis with laser scan of our confocal system. The CoolLED pE-300^white has replaced our old and worn out mercury lamp - we love it for its simplicity in use, and even more for its really bright illumination. Before we had to guess the objects, now we can see them clearly. What a difference!

  Prof. Dr. A. Preiss, University of Hohenheim

• Our customer required a fluorescence light source for their new microscope and had enquired about a metal halide light source unaware of the CoolLED pE-300 Series. Once we had demonstrated the pE-300^white they were immediately impressed with its capabilities. The ability to control the intensity of the light and the fact that it does not involve changing bulbs were major attractions. Through their work with mosquitos they hope to reduce the spread of diseases such as malaria. To do this they need to be able to clearly see which mosquitoes are fluorescing and the CoolLED pE-300^white provides perfect excitation.

  Alan Todd, Microscope Services  Ltd, UK

Research Papers

• Diversity in striatal synaptic circuits arises from distinct embryonic progenitor pools in the ventral telencephalon
• A Custom-Made Device for Reproducibly Depositing Pre-metered Doses of Nebulized Drugs on Pulmonary Cells in vitro
• cAMP-Dependent Signaling Restores AP Firing in Dormant SA Node Cells via Enhancement of Surface Membrane Currents and Calcium Coupling
• hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination
• Nucleo-cytoplasmic shuttling of murine RBPJ by Hairless protein matches that of Su(H) protein in the model system Drosophila melanogaster

White Papers

• Sustainability in the Laboratory: Rethinking Fluorescence Microscopy
• Minimising photodamage during fluorescence microscopy with LED illumination
• Capturing high-speed multi-labelled events with LED illumination systems
• Optimising irradiance for fluorescence microscopy
• Measuring illumination intensity with accuracy and precision

• Energy consumption and cost: LEDs vs Mercury
• Understanding developmental genetics of plants to improve future crop performance
• Improving microbiological testing in a diagnostic laboratory

• LED Microscopy Illumination for Neuroscience
• CoolLED pE-300 Series Leaflet
• CoolLED pE-300 Series Leaflet (Spanish)
• CoolLED pE-300 Series Leaflet (French)
• CoolLED pE-300 Series Quick Start Guide
• CoolLED pE-300 Series Quick Start Guide (Spanish)
• CoolLED pE-300 Series User Manual
• CoolLED pE-300^white & pE-300^ultra Improved Multi-stain Testing
• CoolLED pE-300 Series Declaration of Conformity
• CoolLED pE-300 Series Troubleshooting Guide
• CoolLED pE-300^white Exploded Diagram