The CoolLED pE-300ultra is a highly controllable fluorescence microscopy Illumination System which offers intense, broad-spectrum LED illumination for imaging most common fluorophores. Fast events can be captured with microsecond switching via multiple TTL inputs, and the ability to mount inline excitation filters allows fast, flexible filter configurations. This, when paired with today’s high-performance multi-band filter sets, facilitates imaging traditionally done via a white Light Source and a filter wheel, with all the benefits of LEDs.
LED technology is quickly evolving, and since 2017 our new generation has double the irradiance at the sample plane, and for even higher irradiance is available as a Direct Fit model (which also reduces the cost to buy and run thanks to fewer consumables). With ACT Label certification, it’s even an environmentally friendly option for illumination.
The pE-300ultra is the most controllable member of the pE-300 Series. In addition to the many features of the pE-300lite and the pE-300white, the system offers precise control over wavelength irradiance and shuttering. Until now these benefits have only been accessible to users of high end, highly flexible LED microscope illuminators such as the CoolLED pE-4000.
It allows adjustment of output in 1% steps, giving precise control. The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.
It is now possible to view and image your samples brighter than ever
As part of the award-winning pE-300 Series, the enhanced pE-300ultra now delivers double the irradiance at the microscope sample plane.
Convallaria sample captured using Photometrics Prime camera with 10 ms exposure.
With the new enhanced pE-300ultra, all three channels at full power use just 60 Watts. That is almost a quadruple efficiency increase. This compares to other LED technology that uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology. Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.
Capturing high-speed events
The pE-300ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white Light Source and a filter wheel, with all the benefits of LED – and most excitingly at speeds not previously so affordable. For live cell imaging applications, events can now be captured that might previously have been missed.
Fast multi-channel imaging without pixel shift
Fluorophores can be viewed either individually or in combination, without filter cube changes. This makes the pE-300ultra ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.
Sequence runner for multi-channel excitation
It is now possible to run through a sequence of excitation channels using a camera which has only a single TTL-out, thanks to the Sequence Runner mode. The order of each fluorophore to be captured is defined via the simple Control Pod, with the pE-300ultra Light Source accepting a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the Light Source.
Direct fit or liquid light guide
There are two pE-300ultra configuration options: direct fit or liquid light guide. The Direct Fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables. As a replacement to a metal halide source on a confocal microscope, the Liquid Light Guide model is particularly suitable for pre-aligning samples before scanning commences. In this way, photo bleaching of the sample can be minimised when LED illumination is used. More information on the available variants can be found under specifications .
Award winning technology
CoolLED’s pE-300 Series was the 2017 winner of the “Go Beyond Award” for “Excellence in sustainability in laboratory and other high technology facilities”. The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products, and projects that are advancing sustainable, high-performance facilities.
The pE-300ultra offers:-
- Clean bright illumination across the spectrum – excites common fluorescent stains
- Specify for existing single and multi-band filter sets – no need to buy new filters
- Instant on/off – No shutters required, no warm up or cool down
- Simple to fit, simple to use – no alignment, a once only adjustment
- Stable & repeatable – reliable and consistent results
- Wide range of microscope adaptors – fits most microscopes
- Precise irradiance control in 1% steps (0-100%) – no ND filters required
- Excellent uniformity over field of view – fixed and stable, no alignment necessary
- Long lifetime
- Removable inline excitation filter holders – no moving parts
- Individual channel triggering via TTL in microseconds
- Sequence Runner – sequenced excitation from a single TTL-out
The system comprises a pE-300ultra Light Source, Control Pod, set of three Excitation Filter Holders and Power Supply.
Control pod for operation and access to settings and usage history.
Manual control for instant on/off and irradiance control in 1% steps from 0 – 100%
Individual bands can be selected/deselected and controlled independently as desired
Via global and individual channel TTL for on/off control of selected channels using a BNC connection on the Light Source.
Triggering speed <20 µs at full power
Remote via USB (B type) for imaging software control (see Imaging Software)
Single TTL input to step through user defined sequence.
Triggering speed <20 µs at full power
Choose direct fit or liquid light guide
There are two pE-300ultra configuration options:
Direct Fit: Higher irradiance is possible when directly connecting to a microscope. Select from a range of microscope adaptors which covers all current and most older models. A simple once-only adjustment will allow optimisation to the optical path of the microscope.
Liquid Light Guide: If there is a need to keep the source of illumination remote from the microscope, the Liquid Light Guide variant is available for use with a 3 mm diameter Liquid Light Guide. An optional pE-Universal Collimator and microscope adaptor can also be selected. We now include a Light Source stand for the pE-300ultra Illumination Systems ordered for light guide delivery.
Choose single-band or multi-band variant
DAPI can be excited at either 365 nm or 400 nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted. To accommodate this, the pE-300ultra can be specified in one of two waveband configurations to match existing filter sets within the microscope.
A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365 nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400 nm. The user can specify the configuration which is appropriate for their microscope.
100-240 V a.c. 50/60 Hz, 1.4 A
|pE-300 Series||Light Source||At the wall (includes power supply unit consumption)|
|Standby||2 W||3.3 W|
|1 band (GYR)||20 W||24.4 W|
|2 bands (BLU & GYR)||38 W||44.3 W|
|Full white irradiance (3 bands)||46 W||56.4 W|
pE-300ultra Light Source: 77 mm(w) x 186 mm(d) x 162 mm(h) – Weight 1.4 kg
pE-300ultra Control Pod: 88 mm(w) x 125 mm(d) x 37 mm(h) – Weight 0.32 kg
pE-300ultra Power Supply: 167 mm(w) x 67 m(d) x 35 mm(h) – Weight 0.62 kg
pE-Universal Collimator: 44 mm(w) x 86 mm(d) x 44 mm(h) – Weight 0.17 kg
Environment and Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED’s products have the following benefits:
- Mercury-free and laser-free
- Energy Efficient: 80% less power
- Long lifetime
- No bulb replacements
- Reduced risk of eye damage
- Quiet operation
- No special disposal regulations or issues
Industry-leading 36 months. Read more here.
We chose the CoolLED light source with our live cell imaging system because we need a fluorescence light source that is efficient, easy to use and has a very bright and robust signal for fluorescence – but is also incredibly gentle on cell samples. Our system is designed around a lack of phototoxicity, so when using fluorescence with holotomography, we really need the ideal excitation that keeps cells happy, but shows exactly what is labelled.
Paul Carman, Nanolive
Adopting LED light sources for fluorescence microscopy can help laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps.
Our CoolLED pE-300 Series is also ACT label certified, which stands for accountability, consistency and transparency. Introduced by My Green Lab, this provides an easy way for scientists to measure and compare the environmental impact of a product by reviewing the product itself, its manufacturing process, packaging and disposal. Visit our environment page to find out more.
|Light Source||Emission Colour||Type||CoolLED LED||Chroma||Semrock|
|pE-300ultra SB, pE-300ultra MB||Blue||Single||365 or 400||49053 CoolLED DAPI|
|pE-300ultra SB||Blue||Single||365||39000 AT Dapi||49000 ET Dapi||LED-DAPI-B|
|pE-300ultra SB||Cyan||Single||460||49001 ET eCFP||49013 ET Teal||LED-CFP-A||LED-mTFP-A|
|pE-300ultra SB||Green||Single||460||49002 ET eGFP||39002 AT eGFP||GFP-4050B||GFP-A-Basic|
|pE-300ultra SB||Green||Single||460||49054 CoolLED GFP|
|pE-300ultra SB||Orange||Single||GYR (525-660)||49004 ET Cy3||49005 ET dsRed||TxRed-4040C||LED-TRITC-A|
|pE-300ultra SB, pE-300ultra MB||Red||Single||GYR (525-660)||49055 CoolLED mCherry #1||49056 CoolLED mCherry #2|
|pE-300ultra SB||Red||Single||GYR (525-660)||49008 ET mCherry||39010 AT Tex. Red||YFP-2427B||LED-mCherry-A|
|pE-300ultra SB||Far Red||Single||GYR (525-660)||49006 ET CY5||39007 AT Cy5||Cy5-4040C|
|pE-300ultra MB||Blue||Single||400||49021 eBFP2||49028 ET Dapi 395||LED-DAPI-B|
|pE-300ultra MB||Cyan||Single||460||49001 ET eCFP||49013 ET Teal||LED-CFP-A||LED-mTFP-A|
|pE-300ultra MB||Green||Single||460||49054 CoolLED GFP|
|pE-300ultra MB||Green||Single||460||49002 ET eGFP||39002 AT eGFP||GFP-4050B||GFP-A-Basic|
|pE-300ultra MB||Orange||Single||GYR (525-660)||49004 ET Cy3||49005 ET dsRed||TxRed-4040C||LED-TRITC-A|
|pE-300ultra MB||Red||Single||GYR (525-660)||49008 ET mCherry||39010 AT Tex. Red||YFP-2427B||LED-mCherry-A|
|pE-300ultra MB||Far Red||Single||GYR (525-660)||49006 ET CY5||39007 AT Cy5||Cy5-4040C|
|pE-300ultra MB||Blue-Green-Red||Multiband||400, 460, GYR (525-660)||69401 – ET Triple Multi LED||LED-DA/FI/TX-A|
|pE-300ultra SB||Blue-Green-Red||Multiband||365, 460, GYR (525-660)||69401 – ET Triple Multi LED||LED-DA/FI/TX-A|
|pE-300ultra SB||Green-Red||Multiband||460, GYR (525-660)||GFP/DsRed-A|
|pE-300ultra SB||Green-Red||Multiband||460, GYR (525-660)||59022 ET eGFP/mCherry||FITC/TxRed-A|
|pE-300ultra MB||Blue-Green||Multiband||400, 460||59001v2 ET DAPI/Green FISH|
|pE-300ultra MB||Blue-Orange||Multiband||400, GYR (525-660)||59002v2 ET DAPI/Orange|
|pE-300ultra MB||Blue-Green-Red||Pinkel||400, 460, GYR (525-660)||89084v2 ET DAPI/Green/Orange FISH||LED-DA/FI/TX-3X-B|
|pE-300ultra MB||Green-Red||Pinkel||460, GYR (525-660)||59222 ET eGFP/mCherry||GFP/DsRed-2X-A|
|pE-300ultra MB||Blue-Red||Multiband||400, GYR (525-660)||59003 v2 ET DAPI/Red FISH|
|pE-300ultra SB||Blue-Green-Red||Pinkel||365, 460, GYR (525-660)||LED-DA/FI/TX-3X-B|
|pE-300ultra SB||Green-Red||Pinkel||460, GYR (525-660)||59022 ET eGFP/mCherry||GFP/DsRed-2X-A|
|pE-300ultra MB||Green-Red||Multiband||460, GYR (525-660)||GFP/DsRed-A|
|pE-300ultra MB||Green-Red||Multiband||460, GYR (525-660)||59022 ET eGFP/mCherry||FITC/TxRed-A|
|pE-300-UT-D-SB-YYY-ZZ:||pE-300ultra direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, set of three excitation filter holders (25 mm diameter) and power supply. Single band filter set configuration|
|pE-300-UT-D-MB-YYY-ZZ:||pE-300ultra direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, set of three excitation filter holders (25 mm diameter) and power supply. Multi-band filter set configuration|
|pE-300-UT-L-SB-ZZ:||pE-300ultra Light Source for use with 3 mm Liquid Light Guide. Single Band filter configuration. Includes Light Source, control pod, set of three excitation filter holders (25 mm diameter) and power supply|
|pE-300-UT-L-MB-ZZ:||pE-300ultra Light Source – Multi-Band. For use with 3 mm Liquid Light Guide. Multi-band filter configuration. Includes Light Source, control pod, set of three excitation filter holders (25 mm diameter) and power supply|
|pE-1906||1.5 m long, 3 mm diameter liquid light guide|
|pE-10400-YYY||pE-Universal collimator for use with a single liquid light guide. Includes customer-specified adaptor|
To specify microscope code (YYY) see Adaptors
To specify power cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA
More information is available on the Accessories tab.
- CoolLED pE-300 Series Leaflet
- CoolLED pE-300 Series Quick Start Guide
- CoolLED pE-300 Series User Manual
- CoolLED pE-300ultra Optogenetics Leaflet
- CoolLED pE-300ultra Exploded Diagram
- CoolLED pE-300white & pE-300ultra Improved Multi-stain Testing
- CoolLED pE-300 Series Declaration of Conformity
- CoolLED pE-300 Series Troubleshooting Guide
The CoolLED pE Driver is first required for software integration, and can be downloaded here.
CoolLED Proprietary ZEN Bridge software is available – Contact CoolLED for further information.
The award winning pE-300 Series is a range of LED Illumination Systems for Fluorescence, Optogenetics, Electrophysiology and high speed microscopy applications. It comprises 3 systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra.
The table below is a quick reference guide to the features offered by the pE-300 Series systems.
|Simple to fit and use|
|No bulb replacement|
|Direct or Light Guide delivery options|
|Stable & Repeatable - 10-100% intensity control|
|Filter set compatibility|
|Individual channel intensity control|
|Individual channel selection|
|Integration with imaging software|
|Global remote triggering (TTL, microsecond)|
|Individual channel triggering (TTL, microsecond)|
|Removable inline filter holders|