Rho GTPase signaling and mDia facilitate endocytosis via presynaptic actin

Authors

Oevel K, Hohensee S, Kumar A, et al.

Topic

Neuroscience

Extract

"Following changes in fluorescence were tracked by an inverted Zeiss Axiovert 200 M microscope, equipped with a 40 x oil-immersion EC Plan Neofluar objective (NA 1.30), an EM-CCD camera (Evolve Delta 512) and a pE-300white LED light source (CoolLED). The scanning format was set to 512x512 pixels with 16-bit sampling. eGFP (Excitation: BP470-40; Emission: BP535-50; Zeiss filter set 38) or Cy5 (Excitation: BP640-30; Emission: BP525-50; Zeiss filter set 50) filter sets were used for pHluorin or CypHer assays, respectively. "

Product Type

pE-300^white

Journal

eLife

Year of Publication

2024

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