LED Microscope Illuminator
  • LED Microscope Illuminator
  • pE-300white with Light Guide
  • pE-300white Control Pod



CoolLED's pE-300white offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains.  The pE-300white Light Source can be fitted directly to the microscope or specified with a liquid light guide.  The system is a mercury-free alternative that is safer, more controllable, and repeatable than conventional high-pressure gas discharge illuminators.  Spectral coverage is from the UV (DAPI excitation) to the Red region (Cy5 excitation). The system is perfect for hospital and regular research laboratory environments.

The pE-300white is a compact and simple to use LED illumination unit.  It provides a broad spectrum of illumination,  covering the excitation bands of common fluorescent stains.  Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%.  Remote control is available via USB or TTL trigger.

Throughout 2017 we will be releasing exciting new products and product updates.  The first member of the CoolLED product range to receive the star treatment is the pE-300 Series.

As part of the award winning pE-300 Series, the NEW Enhanced pE-300white  now delivers DOUBLE the intensity at the microscope sample plane.  It allows adjustment of output in 1% steps, giving precise control.  The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.



It is now possible to view and image your samples brighter than ever.

Convaleria taken with Photometrics Prime camera with 10ms exposure.

LED illumination means no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. With the new enhanced pE-300white, all 3 channels at full power use just 46 Watts. That is almost a quadruple efficiency increase!

This compares to other LED technology that uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology.  Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.

CoolLED’s pE-300 Series  was the 2017 winner of the “Go Beyond Award” in the “Products” category for “Excellence in sustainability in laboratory and other high technology facilities”  The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products,and projects that are advancing sustainable, high-performance facilities.

The system comprises a pE-300white Light Source, control pod, and power supply.  It can be specified with a microscope adaptor for direct fit configuration or with a 3mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.

As a replacement to a metal halide source on a confocal microscope, the liquid light guide configuration is particularly suitable for pre-aligning samples before scanning commences.  In this way, photo bleaching of the sample can be minimised when LED illumination is used.

An additional benefit of the pE-300white is that  stains can be viewed either individually or in combination, without filter cube changes.  This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used.  Independent control of the three LED wavebands means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether. For further information on improved multi-stain testing, go to the Downloads tab.


With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300white can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

There are two pE-300white configuration options:

Direct-fit  for connecting to a microscopes – by selecting from a range of microscope adaptors  which covers all current and most older models.  A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide with a fixed 3mm diameter, liquid light guide.  An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.

For further information go to the Downloads tab

The pE-300white offers:-

  • Clean bright illumination across the spectrum – excites common fluorescent stains
  • Specify for  existing single and multi-band filter sets – no need to buy new filters
  • Instant on/off  - No shutters required, no warm up or cool down
  • Simple to fit, simple to use – no alignment, a once only adjustment
  • Stable & repeatable – reliable and consistent results
  • Wide range of microscope adaptors – fits most microscopes
  • Precise intensity control in 1% steps (0-100%) – no ND filters required
  • Excellent uniformity over field of view – fixed and stable, no alignment necessary
  • Long lifetime – expected to exceed 25,000 hours of operating time

DAPI can be excited at either 365nm or 400nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted.  To accommodate this, the pE-300white can be specified in one of two waveband configurations to match with the user’s existing filter sets within the microscope.

A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400nm.  The user can specify the configuration which is appropriate for their microscope.

Power requirements
100-240V a.c. 50/60Hz, 2A

Power consumption
Standby Max 2W
1 band (GYR) at 100% intensity Max 20W
2 bands (BLU & GYR) at 100% intensity Max 38W
White (all 3 bands) at 100% intensity 46W

pE-300white Light Source: 77mm(w) x 186mm(d) x 162(h) - Weight 1.4kg
pE-300white Control Pod: 88mm(w) x 125mm(d) x 37mm(h) - Weight 0.32kg
pE-300white Power Supply: 167mm(w) x 67m(d) x 35mm(h) – Weight 0.62kg

pE-Universal Collimator: 44mm(w) x 86mm(d) x 44mm(h) – Weight 0.17kg

Environment & Safety 

  • Mercury-free
  • Energy Efficient: 80% less power
  • Long lifetime (25,000 operating hours)
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues

System = 24 months extendable by free product registration.  LEDs = 36 months

Control pod for operation and access to settings and usage history.

Manual control for instant on/off and intensity control in 1% steps from 0 – 100%
Individual bands can be selected/deselected and controlled independently as desired

Via single TTL for on/off control of selected channels using a BNC connection on the light source.
Triggering speed <20us

Remote via USB (B type) for imaging software control (see Imaging Software)

Direct-fit to a microscope
Specify from CoolLED’s range of epi-fluorescent or transmitted port microscope adaptors

Liquid Light-Guide
1.5m long, 3mm diameter light-guide.

pE-300-W-D-SB-YYY-ZZ: pE-300white direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Single band filter set configuration
pE-300-W-D-MB-YYY-ZZ: pE-300white direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Multi band filter set configuration
pE-300-W-L-SB-ZZ: pE-300white Light Source for use with 3mm Liquid Light Guide.  Single Band filter configuration.  Includes light source, control pod & power supply
pE-300-W-L-MB-ZZ: pE-300white Illumination System - Multi-Band.  For use with 3mm Liquid Light Guide.  Multi band filter configuration.  Includes light source, control pod & power supply
pE-1906 1.5m long, 3mm diameter liquid light guide
pE-10400 pE-Universal collimator for use with a single liquid light guide. Requires additional microscope adaptor
pE-ADAPTOR-YYY Microscope Adaptor to customer-specified microscope

To specify microscope code (YYY) see Adaptors
To specify power cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

CoolLED pE-300 Series datasheet

CoolLED pE-300 Series User Manual

CoolLED pE-300white  & pE-300ultra Improved Multi-stain Testing

CoolLED pE-300 Series Declaration of Conformity 

pE-10400:   pE-Universal Collimator for use with a single liquid light guide. Requires additional microscope adaptor
pE-ADAPTOR-YYY:   Microscope adaptor to customer-specified microscope
To specify microscope code (YYY) see Adaptors
pE-6501:   The pE-6501 is a USB controlled TTL trigger box which allows seamless integration of the pE-300white into major software packages e.g. cellSens, MetaMorph & uManager.
    Download the leaflet here


"We are absolutely delighted with the pE-300white light source - it is fabulous. We use it as illumination on our Zeiss Axiskop to basically just identify the objects of desire for further analysis with laser scan of our confocal system.

The CoolLED pE-300 has replaced our old and worn out mercury lamp - we love it for its simplicity in use, and even more for its really bright illumination. Before we had to guess the objects, now we can see them clearly. What a difference!"

Prof. Dr. A. Preiss, University of Hohenheim

The CoolLED pE-300white is a perfect fluorescence illumination system for fixed fluorescence samples. We used it on a Nikon E400 upright microscope with four fluorescence channels. With a triple-band filter, it can also be used for simultaneous illumination of three fluorescent proteins. As a system running in a microscope facility, it provides excellent flexibility, enough brightness in all bands to most samples, and is cost-effective, stable, trouble-free, and easy to operate

Dr. Yan Gu, Manager, Analytical and Quantitative Light Microscopy Facility, Genome Damage and Stability Centre, University of Sussex

The lamp is on the microscope and is really impressive. A great product! 

Viki Allan, Professor of Cell Biology, University of Manchester

The CoolLED pE-300white is the perfect fluorescence illumination system for teaching bio-medical students.  It is not only bright enough, but the ability to simultaneously illuminate 3 fluorescent proteins means students can observe structures with better context therefore achieving a more efficient learning outcome.

Urs Ziegler, Ph.D. Head of Center for Microscopy and Image Analysis, University of Zurich

Our imaging facility houses a range of different confocal microscopes from major vendors such as Olympus, Zeiss and Nikon.  All of these systems, including one purchased last year, were supplied with either a mercury arc burner or metal halide light source, used for observation of samples by eye prior to imaging using the confocal laser scanning system.  These light sources suffer many shortcomings, such as the requirement to frequently replace expensive components (burners, light guides), the need for heating/cooling cycles when turning on and off,  safety issues regarding risk of explosion and problems with the disposal of mercury-containing bulbs.  For these reasons we decided to replace all of our confocal epi-illuminators with the CoolLED pE-300white.  Users now have the benefit of immediate on/off with no requirement for warm-up/cool-down cycles, they can fine-tune the intensity of illumination to match their labelling, there is no risk of explosion and there are no problems with the disposal of mercury-containing components.  We have found the CoolLED pE-300white to be highly reliable, simple to operate and work very well with all the standard fluorophores we use including DAPI, Alexa fluor 488, Alexa fluor 568.  To my knowledge there have been no instances where the LED light source has been judged inferior to the illuminator it replaced, with most users appreciating the benefits.  As a facility, the decision to replace our epi-illuminators with LEDs was seen as an investment that should be recovered within 3 years, with the projected lifetime of the LED illuminators considerably exceeding this time. 

Simon Walker, Imaging Facility Manager - Babraham Institute

The pE-300white has been a great asset for a core facility. It has meant less time spent changing bulbs (so less downtime to the user and more productive time for ourselves) and no longer worrying about users turning the light source on and off throughout the day as multiple users come and go.  The controllable intensity has also proved highly popular with users able to set the minimum light level required to scan their samples and minimise the risk of phototoxic damage and bleaching to their specimens.

Peter O'Toole, Head of Imaging & Cytometry - University of York

I just signed off on the installation of our new CoolLED pE-300white,  fitted today on our Leica SP5 confocal. Happy to see the Hg bulb made obsolete! I love it and I’m sure the users will too.

Rosey van Driel, Manager for Transmission Electron Microscopy (TEM), Institute for Frontier Materials, Deakin University, Australia

We are extremely happy with our CoolLED pE-300white System. Integration with our microscope was simple, and the robust system has never needed maintenance or alignment. I am particularly happy with the user interface as it is simple and intuitive.

David Inglis, Senior Lecturer, Dept of Engineering, Macquarie University, Australia

We’ve just bought the CoolLED pE-300white, the little brother to the pE-4000 (on the confocal, we don’t need the more powerful version), it’s really nice.

Dr Martin Spitaler, Head of Imaging Facility, Max Planck Institute of Biochemistry, Germany

We find the system to be excellent, it gives us bright fluorescence and being able to switch the bulb on and off without having to worry about warming up/cooling down is a big advantage over our old system. 

Dr Kat Millen , Lab Manager , University of Cambridge, United Kingdom 


The award winning pE-300 Series is a range of LED Illumination Systems for Fluorescence, Optogenetics, Electrophysiology and high speed microscopy applications. It comprises 3 systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra.

The table below is a quick reference guide to the features offered by the pE-300 Series systems

For more detailed information, please visit the product page by clicking the relevant link below.

 CoolLED's pE-300 Series  was the winner of the "Go Beyond Award" in the “Products” category for “Excellence in sustainability in laboratory and other high technology facilities”  The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products,and projects that are advancing sustainable, high-performance facilities.