Microscope LED light source
  • Microscope LED light source
  • pE-300white with Light Guide

Overview

NOW DOUBLE THE INTENSITY AT THE SAMPLE PLANE

CoolLED’s pE-300lite is a simple white light LED illumination system that offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains.

The pE-300lite Light Source can be fitted directly to the microscope or specified with a liquid light guide. The system is a mercury-free alternative that is safer, more controllable, and repeatable than conventional high-pressure gas discharge illuminators. Spectral coverage is from the UV (DAPI excitation) to the Red region (Cy5 excitation). The system is perfect for hospital and regular research laboratory environments and is affordable within consumables budgets.

The pE-300lite is a simple white light LED illumination unit.  It provides a broad spectrum of illumination,  covering the excitation bands of common fluorescent stains.  Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%.  

Throughout 2017 we will be releasing exciting new products and product updates.  The first member of the CoolLED product range to receive the star treatment is the pE-300 Series.

As part of the award winning pE-300 Series, the NEW Enhanced pE-300lite  now delivers DOUBLE the intensity at the microscope sample plane.  It allows adjustment of output in 1% steps, giving precise control.  The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.

 

 

It is now possible to view and image your samples brighter than ever.

LED illumination means no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. With the new enhanced pE-300lite, this simple white light, at full power uses just 46 Watts. That is almost a quadruple efficiency increase!

This compares to other LED technology that uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology.  Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.

CoolLED’s pE-300 Series  was the winner of the “Go Beyond Award” in the “Products” category for “Excellence in sustainability in laboratory and other high technology facilities”  The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products,and projects that are advancing sustainable, high-performance facilities.

The system comprises a pE-300lite Light Source, control pod, and power supply.  It can be specified with a microscope adaptor for direct fit configuration or with a 3mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.

As a replacement to a metal halide source on a confocal microscope, the liquid light guide configuration is particularly suitable for pre-aligning samples before scanning commences.  In this way, photo bleaching of the sample can be minimised when LED illumination is used.

Print

With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300lite can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

There are two pE-300lite configuration options:

Direct-fit  for connecting to a microscopes – by selecting from a range of microscope adaptors  which covers all current and most older models.  A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide with a fixed 3mm diameter, liquid light guide.  An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.

For further information go to the Downloads tab

The pE-300lite offers:-

  • Simple to Buy
    • Works with everyday fluorophores/stains
    • Affordable through lab consumables budget
    • Broad spectrum - covers UV (DAPI) to Red region (Cy5)
    • Specify for  existing single and multi-band filter sets – no need to buy new filters
  • Simple to Fit
    • Direct fit to the microscope in seconds
    • Wide range of microscope adaptors – fits most microscopes
    • No alignment, a once only adjustment
  • Simple to Use
    • Instant on/off  - No shutters required, no warm up or cool down
    • Stable & repeatable – reliable and consistent results
    • Precise intensity control in 1% steps (0-100%) – no ND filters required
    • Excellent uniformity over field of view – fixed and stable, no alignment necessary
    • Long lifetime – expected to exceed 25,000 hours of operating time
    • No bulb replacement or ongoing consumables

DAPI can be excited at either 365nm or 400nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted. To accommodate this, the pE-300lite can be specified in one of two waveband configurations to match with the user’s existing filter sets within the microscope.

A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400nm. The user can specify the configuration which is appropriate for their microscope.

Power requirements
100-240V a.c. 50/60Hz, 1.4A

Power consumption
2W (standby)
46W (full white intensity)

Dimensions

pE-300lite Light Source: 77mm(w) x 186mm(d) x 162(h) - Weight 1.4kg
pE-300lite Control Pod: 88mm(w) x 125mm(d) x 37mm(h) - Weight 0.32kg
pE-300lite Power Supply: 167mm(w) x 67m(d) x 35mm(h) – Weight 0.62kg

pE-Universal Collimator: 44mm(w) x 86mm(d) x 44mm(h) – Weight 0.17kg

Environment & Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:

  • Mercury-free
  • Energy Efficient: 80% less power
  • Long lifetime (25,000 operating hours)
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues

Warranty
System = 36 months, LEDs = 36 months

Control pod for operation and access to settings and usage history.

Manual:
Manual control for instant on/off and intensity control in 1% steps from 0 – 100%

 

Direct-fit to a microscope
Specify from CoolLED’s range of epi-fluorescent or transmitted port microscope adaptors

Liquid Light-Guide
1.5m long, 3mm diameter light-guide - alternative lengths of liquid light guide are available

 

pE-300-LT-D-SB-YYY-ZZ: pE-300lite direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Single band filter set configuration
pE-300-LT-D-MB-YYY-ZZ: pE-300lite direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, and power supply. Multi band filter set configuration
pE-300-LT-L-SB-ZZ: pE-300lite Light Source for use with 3mm Liquid Light Guide.  Single Band filter configuration.  Includes light source, control pod & power supply
pE-300-LT-L-MB-ZZ: pE-300lite Illumination System - Multi-Band.  For use with 3mm Liquid Light Guide.  Multi band filter configuration.  Includes light source, control pod & power supply
pE-1906 1.5m long, 3mm diameter liquid light guide
pE-10400-YYY pE-Universal collimator for use with a single liquid light guide. Includes Microscope Adaptor to customer-specified microscope

To specify microscope code (YYY) see Adaptors
To specify power cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

CoolLED pE-300 Series Datasheet

CoolLED pE-300 Series Declaration of Conformity 

 

pE-10400-YYY:   pE-Universal Collimator for use with a single liquid light guide. Includes microscope adaptor to customer-specified microscope
pE-ADAPTOR-YYY:   Microscope adaptor to customer-specified microscope

 

To specify microscope code (YYY) see Adaptors

 

The award winning pE-300 Series is a range of LED Illumination Systems for Fluorescence, Optogenetics, Electrophysiology and high speed microscopy applications. It comprises 3 systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra.

The table below is a quick reference guide to the features offered by the pE-300 Series systems

 

For more detailed information, please visit the product page by clicking the relevant link below.

CoolLED's pE-300 Series was the 2017 winner of the "Go Beyond Award" in the “Products” category for “Excellence in sustainability in laboratory and other high technology facilities”  The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products,and projects that are advancing sustainable, high-performance facilities.