• pE-100
  • pE-100light guide
  • pE-100fiber


The pE-100 series is a mercury-free family of compact and simple to use fluorescence LED illumination systems which can be configured to deliver light directly to a microscope, or via a liquid light guide or multimode fiber. Systems can be specified at any one of 20 different LED wavelengths. Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%. Remote control is available via a TTL trigger.

The pE-100 system has been developed for fluorescence applications requiring a single LED excitation wavelength.  It is perfect for clinical applications such as routine screening (e.g. using Auramine for Tuberculosis), for research applications requiring precise intensity control and fast-switching, or for electrophysiology applications where light has to be delivered to a specific location.  The user can select from 20 different LED wavelengths, ranging from the near-UV at 365nm to the near-IR at 770nm.  The system comprises a pE-100 LED Light Source, control pod, and power supply. pE-100 | CoolLED LED Wavelength Graph There are three standard pE-100 configuration options: Direct-fit (pE-100) for connecting to a microscope (epi-port) by selecting from a range of microscope adaptors  which covers all current and most older models.  A simple once only adjustment will allow optimisation to the optical path of the microscope. Liquid Light Guide (pE-100light guide) with a fixed 3mm diameter, liquid light guide Fiber (pE-100fiber) with an SMA connection for accepting multimode fibers.  The pE-100fiber has been designed with efficient coupling into a wide range of multimode fibers.  The pE-100fiber also includes excitation filter holders. Combining pE-100s: Direct fit configuration pE-100 units can be combined using the pE-Combiner for applications requiring a second LED wavelength with independent control and triggering. Liquid light guide or multimode fiber using a fixed two wavelength configuration can be specified.  Two pE-100 Light Sources are combined, providing independent control and triggering. For further information go to the Downloads tab

The pE-100 offers:-

  • Broadest LED spectrum available: 365-770nm – replaces metal halide or mercury
  • Compatible with existing single and multi-band filter sets – no need to buy new filters
  • Instant on/off  - no shutters required, no warm up or cool down
  • Simple to fit, simple to use – no alignment, a once only adjustment
  • Stable & repeatable – reliable and consistent results
  • Wide range of microscope adaptors – fits most microscopes
  • Precise intensity control in 1% steps (0-100%) – no ND filters required
  • Excellent uniformity over field of view – fixed and stable, no alignment necessary
  • Long lifetime – expected to exceed 25,000 hours of operating time

Power requirements 100-240V a.c. 50/60Hz, 0.7A Power consumption 2.2W (standby) 25W (full intensity) Dimensions pE-100 Light Source (direct fit): 60mm(w) x 170mm(d) x 70mm(h) - Weight 0.42kg pE-100light guide Light Source: 52mm(w) x 95mm(d) x 52mm(h) – Weight 0.42kg pE-100fiber Light Source: 76mm(w) x 165mm(d) x 80mm(h) - Weight 0.42kg Combined 2 x pE-100fiber:  190mm(w) x 220mm(d) x 190mm (h) – Weight 1.5kg pE-100 Control Pod: 102mm(w) x 110mm(d) x 50mm(h) - Weight 0.55kg pE-100 Power Supply: 55mm(w) x 95mm(d) x 40mm(h) – Weight 0.19kg

Environment & Safety

  • Mercury-free
  • Energy Efficient: 80% less power
  • Long lifetime (25,000 operating hours)
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues

 Warranty System =  36 months from the date of despatch 

Control pod for operation and access to settings and usage history. Manual: Manual control for instant on/off and intensity control in 1% steps from 0 – 100% Remote: Via single TTL for on/off control using a BNC connection on the control pod.  Triggering speed approx. 150us

244-87-XXX-YYY-ZZ: pE-100 direct fit Light Source, fixed collimating adaptor to customer-specified microscope, remote manual control pod, and power supply
244-87-XXX-L15-ZZ: pE-100light guide Light Source with fixed 3mm diameter, 1.5m long liquid light guide, remote manual control pod, and power supply
pE-100-F-XXX-ZZ: pE-100fiber Light Source, remote manual control pod, SMA fiber connection, and power supply (no fiber provided)
pE-100-F-XXX-XXX-ZZ: Combination of two pE-100fiber Light Sources, remote manual control pod, SMA fiber connection, and power supply. Accepts 25mm diameter excitation filters (fiber and filters not provided)

  A range of fibers is available from CoolLED.  See Accessories To specify wavelength (XXX) see LED Wavelengths To specify microscope code (YYY) see Adaptors To specify power cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

Direct-fit to a microscope Specify a microscope adaptor from CoolLED’s range of epi-fluorescent or transmitted port microscope adaptors Liquid Light Guide 1.5m long, 3mm diameter liquid light guide. Multimode Fiber SMA fitting on the pE-100fiber unit Specify length, numerical aperture (NA), and diameter of fiber Specify termination (e.g. SMA or cleaved end)

CoolLED pE-100 Leaflet

CoolLED pE-100 User Manual Direct LLG

CoolLED pE-100 User Manual Fiber 

Product Videos Introducing CoolLED pE-100 CoolLED pE-100 in Depth Instruction Videos Aligning the pE-100 The pE-Combiner

CoolLED pE-100 Declaration of Conformity 

244-93-XXX-XXX-YYY:   The pE-Combiner offers the ability to combine two pE-100s into a single optical output for dual staining and stimulation applications. Includes an excitation filter holder in each optical path
244-94-XXX-XXX-YYY:   The pE-Combiner offers the ability to combine two pE-100 Light Sources into a single optical output for dual staining and stimulation applications. 
    CoolLED pE-Combiner & Ex -Filters-250  
244-66-YYY:   The pE-Dual Adapt allows two illumination systems to be fitted to a microscope. User can select either illumination system by moving a manual mirror
pE-10400:   pE-Universal collimator for use with a single liquid light guide. Requires additional microscope adaptor
pE-ADAPTOR-YYY   Microscope adaptor to customer-specified microscope

  To specify wavelength (XXX) see LED Wavelengths To specify microscope code (YYY) see Adaptors To specify power cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA   Fibers CoolLED’s pE-100 illumination system can be configured to accept a fiber via either an SMA or FC connector for connecting a wide range of fibers. The user can source the fiber independently or request supply from CoolLED by specifying:- Diameter in um (AAAA) Numerical Aperture in mm (0BB) Length in metres (C) Termination 1 i.e. SMA, FC or C60- cleaved 60mm bare (DDD) Termination 2 i.e. SMA, FC or C60-cleaved 60mm bare (EEE) Product Code: pE-FAAAA-0BB-C-DDD-EEE A range of fibers is available from CoolLED.  See Accessories Examples below:-  

pE-F50-022-2-SMA-SMA   50 um diameter, 0.22 Numerical Aperture (NA), 2m long fiber with two SMA connections
pE-F50-022-2-SMA-C60   50 um diameter, 0.22 Numerical Aperture (NA), 2m long fiber with one SMA connection and one 60mm cleaved end

Contact CoolLED for your specific requirements

"The main benefit of CoolLED's light source is the ability to switch on and off when required and also not having to align or change a bulb every 300 hours. Over the years it will pay for itself when compared to the cost of mercury bulbs."
Kevin MacKenzie, Histology/EM and Imaging Facility, Institute of Medical Sciences, Aberdeen University

"We have had great results using the CoolLED pE-100 on our TB, fungus and bacteria screening microscopes and find the CoolLED LED products make screening quick and easy"
Richard Wills, Microbiology Laboratory Manager at The Royal Berkshire Hospital

"I originally approached various companies with regards to getting an LED light source for my Leica DMLB microscope. The fact that the microscope was old and had a mechanized stage meant that some of the LED systems that we looked at were unsuitable to be fitted. Thankfully the CoolLED system simply slots in where the old mercury bulb unit used to sit. It took less than 5 minutes to fit, and was ready to use once it was switched on. No more waiting for the bulb to get to temperature, no more bulb changing, or re-calibrating of the mirrors before use. Simply marvellous!"
Kam Yim Wong, Immunology Dept, Derriford Hospital, Plymouth UK

CoolLED pE-100 (470 nm) provides very bright and stable excitation light for GCaMP3 imaging purpose. Usage of LED lamp s very easy and convenient, additionally TTL trigger on and off provides some flexibilities.

Chuanqiang Zhang, PhD student, Hans-Berger Department of Neurology, University Hospital Jena