Epifluorescence imaging and optogenetic stimulation for in-vitro electrophysiology
Alessandro Galloni – PHd Student, The Rancz Lab, Francis Crick Institute, London.
Patch-clamp electrophysiology is a widespread method for analyzing the intrinsic properties of neurons and the local connections between them. In recent years, the availability of transgenic mouse lines labelling specific subsets of neurons throughout the brain, has enabled new approaches to analyzing neuronal circuits and studying neuronal diversity across the brain. We are using a CoolLED pE-4000 illumination system coupled to a patch-clamp microscope to visualize tdTomato-labelled layer 5 pyramidal neurons in mouse brain slices and target them for intracellular patch-clamp recordings.
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