Dr Martin Bootman, Reader in Biomedicine, Faculty of Science, Technology, Engineering & Mathematics
Analysing cellular signalling and sub-cellular processes are important aspects of cell biology research. For this we rely heavily on rapid, high resolution fluorescence measurement techniques – including Fura-2 ratiometric calcium imaging and the ability to stably image fluorophores in small cellular compartments.
In this application note, to understand mitochondrial flickers in more detail, we tracked their temporal profile in HEK cells using our widefield fluorescence microscope setup (Zeiss Axio Observer 7 microscope with a Zeiss Axiocam 720) and a CoolLED pE-800fura Illumination System. Exciting the mitochondrial-specific tetramethylrhodamine ethyl ester (TMRE) indicator with the 550 nm LED, we visualised multiple mitochondria in the same cell (Video 1).
CoolLED specialises in LED microscope lighting and, since our team of four introduced the first commercially available LED microscope illuminator in 2006, we have led the way in designing and manufacturing cutting edge LED Illuminators for Microscopes using the latest technology.
