Since LEDs were introduced to microscopy illumination in bioscience research, convincing research groups and imaging facilities of their scope and potential to completely replace metal-halide systems as a suitable replacement for HBO arc lamps has been challenging. But with the recent introduction of full spectrum light units and more advanced systems, LED illumination is becoming the new standard…
…LED illumination has become an option in bright-field microscopy, but it is in the field of fluorescence microscopy that it has made the largest impact. Until the last decade, the high-pressure mercury-vapor arc-discharge lamp was standard with xenon as an alternative for quantitative work; it was about this time that LEDs emerged as an option in fluorescence microscopy. LEDs overcame many of the problems associated with arc lamps, including the repeated exchange of bulbs and subsequent alignment, restart times, fluctuations in intensity after start-up, and intensity drop-off over time…
Click here to read the full article by our very own Dr Robert Hartley!