Since LED illumination systems for fluorescence microscopy arrived on the market, what is often known as “illumination intensity” has been a hot topic – and rightly so. The amount of light reaching the sample significantly impacts the brightness and contrast of fluorescence microscopy images and therefore data quality.
However, the topic has been surrounded by complex, confusing and sometimes inaccurate measurements and terminology. Even the term “intensity” is inaccurate.
A key issue has been an industry-wide lack of a standardised approach to measure illumination, and in our new white paper we are setting out to change this. We introduce a protocol whereby microscopists can quantify the amount of light at the sample plane and therefore directly compare systems to find their ideal solution.
Read our new white paper to find out:
- What is meant by illumination intensity and why we instead use the term irradiance
- Why quantifying irradiance is crucial for fluorescence microscopy
- Where irradiance should be measured and why
- How to accurately and precisely measure irradiance with our new method