The increased awareness of photodamage in fluorescence microscopy experiments has highlighted the importance of controlling sample illumination.
Photodamage in fluorescence microscopy occurs when high-intensity light used to excite fluorophores generates reactive oxygen species, leading to the degradation of both the fluorophores and the biological specimen. This damage compromises image quality and can distort experimental results by altering the biological processes under observation.
Using CoolLED LED microscopy light sources significantly reduces photodamage. LEDs emit light at lower intensities and with minimal heat production, thus decreasing the generation of reactive oxygen species. Additionally, our systems offer precise control over wavelength and intensity, further minimising unnecessary exposure and preserving the integrity of the sample.
Take a look at our white paper and learn how to apply LED microscopy illumination to protect samples against the effects of both photobleaching and phototoxicity.
Application notes & insights from experts such as Dr Alison North
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Why CoolLED?
Simple to fit and use, CoolLED Illumination Systems are the ideal upgrade for modern laboratories
Quick to interchange between major microscope brands
Broad spectrum (DAPI – Cy5)
Liquid light guide or direct fit
World-renowned support
Industry-leading warranty
Find your local CoolLED Reseller
Wherever you are, the CoolLED product range is available through our worldwide Resellers, including the major microscope manufacturers (Olympus, Nikon, Leica and Zeiss).
To discuss your system requirements, follow the link to find your local Reseller, or speak to your existing microscope supplier.
