The Mesolens is a giant microscope objective designed for computer data acquisition rather than the human eye. It arose from a realization in the early days of confocal microscopy that confocal images could not be obtained of large specimens, because the available low magnification objectives had too poor a resolution in depth.
The University of Strathclyde have created the Mesolens with an unprecedented numerical aperture of nearly 0.5 at a magnification of 4x. This results in a field size of 6 mm, with a working distance of 3 mm, and the possibility to resolve sub-cellular detail throughout this large volume in x, y and z.
New Mesolens publication has now been accepted and published by eLife – “A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout”
Click here for further information.
Bovine pulmonary artery endothelial cells with MitoTracker Red CMXRos (red), Alexa Fluor 488 Phalloidin (green), and DAPI (blue) – slide commercially available as ‘Fluocells slide #1’ from Thermofisher Scientific. Imaged with 20ms camera exposure sequential widefield epi-fluorescence mode using a CoolLED pE-4000 illuminator