Unlocking High-Speed Multi-Colour Imaging with Cost-Effective LED Automation
In the realm of microscopy, achieving high temporal resolution and high contrast multi-colour imaging often demands an expensive setup. However, the innovative Sequence Runner function offers a cost-effective solution by triggering illumination directly from the camera via TTL. This breakthrough technology is especially valuable for high-speed multi-colour fluorescence imaging.
What is Sequence Runner?
Sequence Runner operates seamlessly with a Global TTL input in compatible CoolLED Illumination Systems. It empowers users to define a customised LED sequence, with LED channel selection and intensity predetermined during the setup. Each LED in the sequence responds to a TTL signal, allowing for precise synchronisation with the camera. This approach revolutionises microscopy illumination with its simplicity and cost-effectiveness.
The advantages of Sequence Runner extend beyond affordability:
- It provides a straightforward method for high-speed automation, transforming manual microscopes into automated imaging systems capable of <7 µs LED triggering in some setups.
- The precise synchronisation with the camera minimises phototoxicity and photobleaching, preserving the integrity of samples and lengthening time-lapse studies.
- Its cost-effectiveness is highlighted by the primary components required: a compatible CoolLED Illumination System and a camera with TTL-out capability.
Which Illumination Systems include Sequence Runner?
For users seeking the integration of Sequence Runner, it comes pre-built into several CoolLED Illumination Systems, including the pE-800, pE-800fura, pE-400max, pE-300ultra, and pE-340fura models.
How to Configure Sequence Runner
Configuring Sequence Runner is a straightforward process, and depends on which Illumination System is in use:
- Nikon NIS Elements AR software (including the Triggered Acquisition module): pE-800, pE-800fura, pE-300ultra, and pE-340fura.
- Manual control pod: pE-300ultra, pE-340fura, and pE-400max
- LightBridge virtual control pod: pE-800 Series, pE-400max
In conclusion, Sequence Runner stands as a beacon of innovation, making high-speed, multi-colour fluorescence imaging accessible without breaking the bank. Its compatibility with various CoolLED Illumination Systems and straightforward configuration options make it a versatile and valuable tool in the realm of microscopy.