At Eötvös Lóránd University we investigate activity-dependent forms of synaptic plasticity and homeostatic regulation of intrinsic excitability of mouse hippocampal neurons. Using whole-cell patch clamp technique we can record the membrane potential of channelrhodopsin-transfected single neurons during optical stimulation. Here we demonstrate the voltage responses of such neurons under stimulation with flickering light pulses delivered by the CoolLED pE-4000 device. The recordings were performed in dissociated mouse embryonal hippocampal cultures, isolated on E18 and cultivated up to 15 days in vitro.
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