CoolLED will launch the pE-340fura , a fast controllable LED illumination system for ratiometric calcium imaging, THIS MONTH!
Peter Tinning of The University of Strathclyde has been working in partnership with CoolLED to develop LED technology in fluorescence and his paper entitled “A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric calcium imaging of live cells with better than 5 nM precision” has now been published in the Journal of Microscopy.
(TINNING, P. W., FRANSSEN, A. J. P.M., HRIDI, S. U., BUSHELL, T. J. and MCCONNELL, G. (2017), A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. Journal of Microscopy. doi:10.1111/jmi.12616).
Intracellular calcium plays an important role in many biological processes including the release of neurotransmitters in the brain, triggering insulin release in the pancreas, and the regulation of muscle contractions. Measuring the response of calcium concentration in response to external stimuli is critical in determining the role of calcium in biological processes. Use of ratiometric calcium indicators, such as Fura-2, allows quantification of intracellular calcium concentration but relies on efficient excitation or detection at two separate wavelengths. This work describes the use of a bespoke 340/380 nm LED illumination system for Fura2 calcium imaging.
340 and 380 images of 1 µM Fura-2 AM loaded into tsA-201 cells
Click here for further information on CoolLED’s pE-340fura