Visualizing the internalization and biological impact of nanoplastics in live intestinal organoids by Fluorescence Lifetime Imaging Microscopy (FLIM)

Authors

Irina A Okkelman, Hang Zhou, Sergey M Borisov, Angela C Debruyne, Austin E Y T Lefebvre, Marcelo Leomil Zoccoler, Linglong Chen, Bert Devriendt, Ruslan I Dmitriev

Topic

Biophotonics, Cell Biology, Materials Science, Time-Lapse Microscopy

Extract

"Widefield fluorescence microscopy was performed on an inverted fluorescence microscope IX81 (Olympus-Evident), equipped with motorized Z-axis control, CoolLED pE4000 (16 channels, 365-770 nm), ORCA-Flash4.0LT+ (Hamamatsu) cMOS camera, temperature controlled stage (OkoLab), and air objectives 4x/0.13 UPlanFLN, 10x/0.3 UPlanFLN, 40x/0.6 LUCPlanFLN, water immersion objective 60x/1.0 LUMPLFLN, CellSens Dimension v.3 software as described previously."

Product Type

pE-4000

Journal

Light Science & Applications

Year of Publication

2025

Read the full research paper here

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