Up-regulated expression of two-pore domain K+ channels, KCNK1 and KCNK2, is involved in the proliferation and migration of pulmonary arterial smooth muscle cells in pulmonary arterial hypertension

Authors

Shima N, Yamamura A, Fujiwara M, et al.

Topic

Calcium Imaging, Cell Biology

Extract

"measurements were performed using the fluorescence imaging system equipped with an ECLIPSE Ti2 inverted microscope, a S FL objective lens (20×/0.75), NIS-Elements imaging software (Nikon), a pE-340fura LED illuminator (CoolLED, Hampshire, UK), and a C9100-12 EM-CCD digital camera (Hamamatsu Photonics, Hamamatsu, Japan). PASMCs were illuminated at 340-/380-nm wavelengths, and the fluorescent emissions (510/80 nm) were obtained every 5 s. The fura-2 signal is presented as the fluorescence ratio (F340/F380). Standard HEPES-buffered solution was used as an extracellular solution."

Product Type

pE-340^fura

Journal

Front Cardiovasc Med.

Year of Publication

2024

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