The paraventricular thalamus provides a polysynaptic brake on limbic CRF neurons to sex-dependently blunt binge alcohol drinking and avoidance behavior in mice

Authors

Levine et al.

Topic

Calcium Imaging, Neuroscience

Extract

"GCaMP6s was excited with 35% 470 nm LED (CoolLED) at a frequency of 1 Hz for 10 s every min to minimize photobleaching, and videos were acquired at a frame rate of 10 Hz with an optiMOS monochrome camera (QImaging, Surrey, British Columbia, Canada) and MicroManager 1.4 software, across the entire experiment including a five min baseline, 10 min bath application of CNO (10 μM), and 10 min washout period.
"

Product Type

pE-100

Journal

Nature Communications

Year of Publication

2021

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