Authors

Olivia B. Levine,1 Mary Jane Skelly,2,9 John D. Miller,2 Jean K. Rivera-Irizarry,1 Sydney A. Rowson,2 Jeffrey F. DiBerto,3,4 Jennifer A. Rinker,5,6,7 Todd E. Thiele,8 Thomas L. Kash,3,4 and Kristen E. Pleilcorresponding author1,2

Topic

Calcium Imaging, Neuroscience

Extract

"GCaMP6s was excited with 35% 470 nm LED (CoolLED) at a frequency of 1 Hz for 10 s every min to minimize photobleaching, and videos were acquired at a frame rate of 10 Hz with an optiMOS monochrome camera (QImaging, Surrey, British Columbia, Canada) and MicroManager 1.4 software, across the entire experiment including a five min baseline, 10 min bath application of CNO (10 μM), and 10 min washout period.
"

Product Type

pE-100

Journal

Nature Communications

Year of Publication

2021