The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts

Authors

Jagoda Gorzkowska, Wiktoria Kozak, Sylwia Bobis-Wozowicz, Ivan Cherepashuk, Zbigniew Madeja, Sławomir Lasota

Topic

Cell Biology

Extract

"Imaging was performed 48–72 h after transient transfection or 24 h after reseeding of GFP-expressing cells, using a Leica DMI8 inverted fluorescence microscope equipped with a DFC7000 GT monochromatic CCD camera, a dry HCX APO U-V-I 40×/0.75 objective, a GFP-optimized filter set including QUAD filter cube and fast filter wheels at FITC position (all from Leica, Wetzlar, Germany), and a CoolLED pE-4000 LED illuminator (CoolLED Ltd., Andover, UK) set at 490 nm diode."

Product Type

pE-4000

Journal

Cell Commun Signal

Year of Publication

2025

Read the full research paper here

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