Authors

Prevedel, R., Yoon, Y.-G., Hoffmann, M., Pak, N., Wetzstein, G., Kato, S., … Vaziri, A.

Affiliations

Research Institute of Molecular Pathology, Vienna, Austria. Max F. Perutz Laboratories, University of Vienna, Vienna, Austria. Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), University of Vienna, Vienna, Austria. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA. MIT Media Lab, MIT, Cambridge, Massachusetts, USA. Department of Mechanical Engineering, MIT, Cambridge, Massachusetts, USA. Department of Biological Engineering, MIT, Cambridge, Massachusetts, USA. Department of Brain and Cognitive Sciences, MIT, Cambridge, Massachusetts, USA. McGovern Institute, MIT, Cambridge, Massachusetts, USA.

Application Area

Neuroscience

Calcium Imaging

Abstract

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

Extract

… “equipped with an LED excitation light source (λ = 465 nm, 300 mW, CoolLED) and a standard”…

Product Associated Features

pE-100: A range of compact, simple-to-use, single wavelength illumination systems for screening fluorescence.

Diascopic Technique

-

Live Cell Issues

Photobleaching

Product Type

pE-100

Journal

Nature Methods

Year of Publication

2014

Country of Publication

Austria