Authors
Prevedel, R., Yoon, Y.-G., Hoffmann, M., Pak, N., Wetzstein, G., Kato, S., … Vaziri, A.
Affiliations
Research Institute of Molecular Pathology, Vienna, Austria. Max F. Perutz Laboratories, University of Vienna, Vienna, Austria. Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), University of Vienna, Vienna, Austria. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA. MIT Media Lab, MIT, Cambridge, Massachusetts, USA. Department of Mechanical Engineering, MIT, Cambridge, Massachusetts, USA. Department of Biological Engineering, MIT, Cambridge, Massachusetts, USA. Department of Brain and Cognitive Sciences, MIT, Cambridge, Massachusetts, USA. McGovern Institute, MIT, Cambridge, Massachusetts, USA.
Application Area
Neuroscience
Calcium Imaging
Abstract
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
Extract
… “equipped with an LED excitation light source (λ = 465 nm, 300 mW, CoolLED) and a standard”…
Product Associated Features
pE-100: A range of compact, simple-to-use, single wavelength illumination systems for screening fluorescence.
Diascopic Technique
-
Live Cell Issues
Photobleaching
Product Type
pE-100
Journal
Nature Methods
Year of Publication
2014
Country of Publication
Austria
