Optogenetic silencing by combining a rhodopsin cyclase with an engineered cGMP-gated potassium channel

Authors

Anika Spreen, Nidish Ponath Sadanandan, Martin Winfried Schneider, Enrico Kuehn, Andries Napo Leemisa, Roberta De Zio, Niklas Meyer, Wayne Busse, Bela Erlinghagen, Lea Adenauer, Thoralf Opitz, Wolfgang Bönigk, Enrico Schiewer, Jonas Heer, Dietmar Schmitz, Johannes Vierock, Heinz Beck, Peter Hegemann, Herwig Baier, Franziska Schneider-Warme, Yinth Andrea Bernal Sierra, Reinhard Seifert

Topic

Optogenetics

Extract

"Light pulses at 525 nm were delivered using a CoolLED pE-4000 system (CoolLED, Andover, UK).

Activation light was provided by a CoolLED pE-800 illumination system and directed to the sample via a DAPI (4′,6-diamidino-2-phenylindole)/FITC (fluorescein isothiocyanate)/Cy3/Cy5/Cy7 Penta LED HC Filter Set (AHF Analysetechnik, Germany)"

Product Type

pE-800, pE-4000

Journal

Sci Adv.

Year of Publication

2025

Read the full research paper here

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