MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope

Authors

Schmidt et al.

Topic

Biophotonics, Neuroscience

Extract

"The optical setup (Fig. 1a, Supplementary Figs. S1, S4, S7, and Supplementary Notes 1 and 2) is based on a common fluorescence microscope (IX83, Olympus) that provides routinely needed features such as compatibility with standard stages (U-780.DOS, Physik Instrumente), sample holders (P-545.SH4, Physik Instrumente), brightfield and epifluorescence illumination (CoolLED pE-4000, CoolLED) with filter sets and eyepieces for quickly checking large ROIs in the sample."

Product Type

pE-4000

Journal

Nature Communications

Year of Publication

2021