MINFLUX microscopy resolves subunits of the cardiac ryanodine receptor and its 3D orientation in cells

Authors

Alexander H Clowsley, Anna Meletiou, Radoslav Janicek, Alexandre F E Bokhobza, Evelina Lučinskaitė, Gabriela Bleuer, Isabelle Jansen, Peter P Jones, William E Louch, Christian Soeller

Topic

Biophotonics, Cell Biology, Molecular Biology

Extract

"For widefield fluorescence, a tuneable LED-light source (CoolLED, Andover) was used to aid cell selection and confirm proximity of 200 nm red fluorescent beads (fiducials) within the imaging region.
Beads were excited using an LED light source (pE4000 CoolLED, Andover) emitting light nominally at 635 nm towards a Cy5 dichroic (Semrock, FF650) and the resulting fluorescence propagated through an appropriate emission filter (Semrock, LP02-647RU) towards the splitter device and camera."

Product Type

pE-4000

Journal

Nature Communications

Year of Publication

2025

Read the full research paper here

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