Light-Regulated Agonists Spatiotemporally Activating the Vitamin D Receptor Mitigate Psoriasis-like Inflammation in Mice without Inducing Hypercalcemia

Authors

Xavier Rovira, Alfonso Espada, Carme Serra, Juanlo Catena, Marc Lopez-Cano, Silvia Panarello, Elisabet Perez-Albaladejo, Howard Broughton, Leticia Cano, Hans Ajieren, Sadid Khan, Paula Alvarez-Montoya, Lourdes Muñoz, Joan Font, Ana Trapero, Pablo Rivero, Yanrong Li, Donghui Ma, Xianglin Yin, Yanfei L Ma, Jeffrey A Dodge, Mingji Dai, Pedro Irazoqui, Francisco Ciruela, Venkatesh Krishnan, Amadeu Llebaria

Topic

Chemistry

Extract

"Light was applied with a CoolLED pe-4000 at 50% intensity that corresponded to 0.75 mW/mm2 for the light at 365 nm and 0.56 mW/mm2 for the light at 470 nm; 10% intensity corresponded to 0.15 mW/mm2 for the light at 365 nm and 0.12 mW/mm2 for the light at 470 nm; and 5% intensity corresponded to 0.06 mW/mm2 for the light at 365 nm and 0.05 mW/mm2 for the light at 470 nm.

PSS state areas were determined at 21 °C by HPLC-MS after 3 min illumination (365/525 nm) of a 60 μM sample with a CoolLED pE-4000 in the aqueous buffer used for the pharmacological experiments (0.6% DMSO) without DTT and BSA

Dose–response curves of compound PhotoVDRM in the dark (black line) and after illumination with increasing light power (2, 5, 10, 20 and 100%) of the CoolLED pE-4000 at 365 nm for 5 s (intensity gradient of violet indicates increasing light power)."