IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals

Authors

A. A. Galiakberova,corresponding author1,2 A. M. Surin,3,4 Z. V. Bakaeva,3,5 R. R. Sharipov,4 Dongxing Zhang,6 D. A. Dorovskoy,6 K. M. Shakirova,1 A. P. Fisenko,3 and E. B. Dashinimaev1,6,7

Topic

Calcium Imaging, Neuroscience

Extract

"Measurements of changes in [Ca2+]i and [Na+]i were performed using an image analysis system based on a Nikon Ti2 microscope (Japan), an LED-based illumination system (PE-340-fura, CoolLED, USA), a triple-band beam-splitter mirror DM468/526/596, a set of excitation filters 340 ± 13 and 387 ± 6 nm for Fura-2 and SBFI, and 442 ± 21 nm for GCaMP6s.
"

Product Type

pE-340^fura

Journal

Neurochemical research

Year of Publication

2021

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