Hyun Jong Kim,1,2 Seorin Park,1 Hui Young Shin,1 Yu Ran Nam,2 Phan Thi Lam Hong,1,2 Young-Won Chin,3 Joo Hyun Nam,corresponding author1,2 and Woo Kyung Kim
1 Department of Physiology, Dongguk University College of Medicine (Republic of Korea); 2 Channelopathy Research Center (CRC), Dongguk University, Goyang (Republic of Korea); 3 College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University (Republic of Korea); 4 Department of Internal Medicine Graduate School of Medicine, Dongguk University (Republic of Korea)
Calcium Imaging, Immunology
As one of the main components of mangosteen (Garcinia mangostana), a tropical fruit, α-mangostin has been reported to have numerous pharmacological benefits such as anti-cancer, anti-inflammatory, and anti-allergic effects through various mechanisms of action. The effects of α-mangostin on intracellular signaling proteins is well studied, but the effects of α-mangostin on ion channels and its physiological effects in immune cells are unknown. Generation of intracellular calcium signaling is a fundamental step for T cell receptor stimulation. This signaling is mediated not only by the ORAI1 calcium channel, but also by potassium ion channels, which provide the electrical driving forces for generating sufficient calcium ion influx. This study investigated whether α-mangosteen suppress T cell stimulation by inhibiting ORAI1 and two kinds of potassium channels (Kv1.3 and KCa3.1), which are normally expressed in human T cells.
Calcium signals were induced in the attached cells and the fluorescence was measured using an illuminator (pE-340 fura, CoolLED, Andover, UK) and recorded using a camera (sCMOS pco.edge 4.2, PCO, Kelheim, Germany).
Product Associated Features
The 340 nm and 380 nm LED illumination system provides the optimum excitation wavelengths for Fura-2-based calcium imaging, allowing high-precision, stable, high-throughput imaging with video-rate time resolution.
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