Authors
Tim Vervliet, Jens Loncke, Marko Sever, Karan Ahuja, Chris Van den Haute, Tomas Luyten, Grace E Stutzmann, Catherine Verfaillie, Tihomir Tomašič, Geert Bultynck
Topic
Biophotonics, Time-Lapse Microscopy
Extract
"Excitation was performed at 470 nm using a CoolLed pR-4000 (CoolLed).
Only mCherry positive cells were measured using the above Nikon microscope equipped with an additional CoolLed pE-340Fura system, a 409 nm blocking edge BrightLine® long-pass filter (Semrock) and a 595/31 emission filter (Semrock).
A CoolLed pR-4000 was used to excite at 380 nm, 470 nm and 580 nm measuring emission at 440 nm, 520 nm and 580 nm for BFP (or Hoechst), FITC and rhodamine signals respectively via a pco.edge 4.2bi sCMOS camera."
Product Type
Journal
Nature Communications
Year of Publication
2026
