Hif-2α programs oxygen chemosensitivity in chromaffin cells

Authors

Prange-Barczynska M, Jones HA, Sugimoto Y, Cheng X, Lima JD, Ratnayaka I, Douglas G, Buckler KJ, Ratcliffe PJ, Keeley TP, Bishop T.

Topic

Calcium Imaging, Cell Biology

Extract

"Tissues were imaged in a custom-built gravity-based perfusion system, with a wide-field Leica DMi8 microscope (Leica Microsystems CMS), configured with a pE340fura light source (CoolLED), a GFP filter cube (excitation filter 470 ± 20 nm, 495 nm dichroic mirror, emission filter 525 ± 25 nm), and a monochrome Prime 95B camera (Photometrics). "

Product Type

pE-340^fura

Journal

J Clin Invest.

Year of Publication

2024

Custom Microscopy Illumination

Need something a little different?

For manufacturers who wish to gain a competitive advantage through bright, stable and controllable illumination, CoolLED’s world-renowned LED technology is available in customised configurations.

Streamlined development using proven off-the-shelf technology
Professional support
Market-leading warranty
‘Try before you buy’ option
Trusted experts in LED illumination technology since 2006

LEARN MORE

Sustainability

Did you know that LED illumination systems help laboratories reduce their carbon footprint, protecting laboratory staff along the way?

Toxic mercury-based microscope illuminators are bad news for the environment, draw a lot of power and have a short lifetime.

LED illumination systems are cleaner, have a long lifetime and use less power. Our pE-300 Series is also ACT label certified and is a natural choice for labs who want to play their part in helping the environment.