Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel

Authors

A Monmeyran 1, W Benyoussef 1, P Thomen 1 2, N Dahmane 3, A Baliarda 3, M Jules 3, S Aymerich 3, N Henry 4

Affiliations

1Sorbonne Université, CNRS, Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, F-75005, Paris, France.
2Université Côte d'Azur, CNRS UMR 7010, Institut de Physique de Nice, Parc Valrose, 06108, Nice, France.
3Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
4Sorbonne Université, CNRS, Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, F-75005, Paris, France. [email protected]

Topic

Microbiology

Abstract

Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory setup for the real-time monitoring of community development. We demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians, and Rhodocyclus sp.) form a dynamic community that deterministically reaches its equilibrium after ~30 h of growth. We reveal the emergence of complexity in this simplified community as reported by an increase in spatial heterogeneity and non-monotonic developmental kinetics. Importantly, we find interspecies interactions consisting of competition for resources-particularly oxygen-and both direct and indirect physical interactions. The simplified experimental model opens new avenues to the study of adherent bacterial communities and their behavior in the context of rapid global change.

DOI: https://dx.doi.org/10.1038%2Fs41522-021-00233-4

Extract

Fluorescence acquisitions were performed using either the green channel filters for GFP and FAST:HBR-2,5-DM (Ex. 482/35, DM 506 Em. FF01-536/40), or the red filter for m-Cherry (Ex 562/40 nm DM 593 Em. 641/75). Excitation was performed using an LED box (CoolLed pE-4000).

Product Associated Features

The pE-4000 Universal Illumination System offers 16 selectable wavelengths from 365 - 770 nm, making it a highly flexible illuminator covering a wide variety of fluorophores

Product Type

pE-4000

Journal

NPJ biofilms and microbiomes.

Year of Publication

2021

Country of Publication

USA