Authors

Craig, M. T., & McBain, C. J

Affiliations

Program in Developmental Neurobiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.

Application Area

Optogenetics

Electrophysiological Techniques

Abstract

Information processing in neuronal networks relies on the precise synchronization of ensembles of neurons, coordinated by the diverse family of inhibitory interneurons. Cortical interneurons can be usefully parsed by embryonic origin, with the vast majority arising from either the caudal or medial ganglionic eminences (CGE and MGE). Here, we examine the activity of hippocampal interneurons during gamma oscillations in mouse CA1, using an in vitro model where brief epochs of rhythmic activity were evoked by local application of kainate. We found that this CA1 KA-evoked gamma oscillation was faster than that in CA3 and, crucially, did not appear to require the involvement of fast-spiking basket cells. In contrast to CA3, we also found that optogenetic inhibition of pyramidal cells in CA1 did not significantly affect the power of the oscillation, suggesting that excitation may not be essential for gamma genesis in this region. We found that MGE-derived interneurons were generally more active than CGE interneurons during CA1 gamma, although a group of CGE-derived interneurons, putative trilaminar cells, were strongly phase-locked with gamma oscillations and, together with MGE-derived axo-axonic and bistratified cells, provide attractive candidates for being the driver of this locally generated, predominantly interneuron-driven model of gamma oscillations.

Extract

… “experiments, we used whole-field illumination with 580 nm light with a CoolLED pE- 4000 (CoolLED)”

Product Associated Features

pE-4000: The universal LED illumination system for research fluorescence, with the broadest spectrum of illumination available from 16 selectable wavelengths, and extensive functionality that sets a new standard for industry.

Diascopic Technique

-

Live Cell Issues

-

Product Type

pE-4000

Journal

The Journal of Neuroscience

Year of Publication

2015

Country of Publication

USA