Excitation and electroporation in genetically engineered excitable S-HEK cells exposed to electric pulses of different durations

Authors

Tina Batista Napotnik, Tina Cimperman, Lea Rems

Topic

Electrophysiology

Extract

"For monitoring AP and changes in TMV, cells were exposed to electric pulses under the microscope Thunder Imager Live Cell system for fluorescence microscopy with DMi8 inverted fluorescent microscope, deep-cooled 4.2 MP sCMOS Leica DFC9000 Gt fast camera, a system of 8 LED diodes (LED8), and a UV light source (CoolLED pE340fura) (all from Leica Microsystems GmbH, Wetzlar, Germany).

Cells were observed under Leica Thunder Imager Live Cell system with 63 × objective. They were illuminated with UV light source (CoolLED pE340fura, Leica) at two excitation wavelengths (340 nm and 380 nm). "

Product Type

pE-340^fura

Journal

Scientific reports

Year of Publication

2025

Read the full research paper here

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