Authors

Blum, Katrin S. Proulx, Steven T. Luciani, Paola Leroux, Jean Christophe Detmar, Michael

Affiliations

Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich.

Application Area

Immunology and tissue regeneration

General Fluorescence Microscopy

Abstract

Knowledge about the mechanisms of regeneration of the lymphatic vasculature after surgical trauma is essential for the development of strategies for the prevention and therapy of lymphedema. However, little is known about the alterations of lymphatic flow directly after surgical trauma. We investigated lymphatic function in mice using near-infrared imaging for a period of 4 weeks after surgeries that mimic sentinel lymph node biopsy (SLNB) or axillary lymph node dissection (ALND), by removal of the popliteal lymph node (LN) alone or together with the popliteal fat pad, respectively. SLNB-like surgery did not cause changes in lymphatic drainage in the majority of cases. In contrast, lymphatic drainage impairment shown by collecting vessel rupture, dermal backflow and rerouting of lymph flow via collateral vessels were observed after ALND-like surgery. All collateral vessels drained to the inguinal LN. These results indicate that less invasive surgery prevents lymphatic decompensation. They also reveal the development and maturation of collateral lymphatic vessels after extensive surgical trauma, which reroute the flow of lymph towards a different LN. These findings might be helpful for the development of strategies to prevent and/or treat post-surgical lymphedema.

Extract

… “a high-powered light emitting diode (LED) system with illumination at 635 nm (CoolLED, Andover, UK) and”…

Product Associated Features

pE-100: A range of compact, simple-to-use, single wavelength illumination systems for screening fluorescence.

Diascopic Technique

-

Live Cell Issues

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Product Type

pE-100

Journal

Breast Cancer Research and Treatment

Year of Publication

2013

Country of Publication

Switzerland