Dynamics of agonist-evoked opioid receptor activation revealed by FRET- and BRET-based opioid receptor conformation sensors

Authors

Sina B Kirchhofer, Claudia Kurz, Lorenz Geier, Anna-Lena Krett, Cornelius Krasel, Moritz Bünemann

Topic

Biophotonics, Molecular Biology, Neuroscience

Extract

"Single-cell FRET measurements describing the activation and deactivation kinetics were measured at an inverted microscope (Eclipse Ti2-E, Nikon, Japan) equipped with a 100x oil-immersion objective (Plan Apo 100x Lambda/1.45 Oil/0.17 WD 0.13, Nikon), LED light source (CoolLED pE4000, CoolLED, Andover, UK) and a high-performance camera (Prime 95B Scientific CMOS, Teledyne Photometrics, Tucson, Arizona, USA)."

Product Type

pE-4000

Journal

Communications Biology

Year of Publication

2025

Read the full research paper here

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