Authors
Jose Ernesto Canton-Josh et al.
Topic
Electrophysiology, Neuroscience, Optogenetics
Extract
"For UBC recordings, cells expressing tdT in a Drd1-Cre; tdT cross were targeted using epifluorescence illumination from a CoolLED pe4000 system (CoolLED Ltd., Andover, UK).
To stimulate ChR2 in UBCs, we used a train of light pulses (1 ms, 10–20 Hz, 20 pulses, 460 nM, 2–5 mW) delivered through a ×60 water-immersion objective (Olympus, Tokyo, Japan) with CoolLED PE4000 widefield illumination (CoolLED Ltd., Andover, UK).
During every trial, we delivered an optogenetic stimulus train (1 ms, 100 Hz, 100 pulses, 460 nM, 2–5 mW) through a ×60 water-immersion objective (Olympus, Tokyo, Japan) with CoolLED illumination PE4000.
During every trial, we delivered an optogenetic stimulus train (1 ms, 39 Hz, 200 pulses, 460 nM, 1–2 mW) through a ×60 water-immersion objective (Olympus, Tokyo, Japan) with CoolLED illumination PE300, with a 20 s ITI.
For optogenetic experiments, the same imaging parameters as for electrical stimulation experiments were used. Instead of electrical stimulation, CoolLED pe300 (CoolLED Ltd., Andover, UK) was used to activate ChR2 (200 pulses at 39 Hz, 2ms pulses, 460 nm, 1–2 mW). "
Product Type
pE-4000, pE-300ultra, pE-300white, pE-300lite
Journal
eLife
