Authors
Michael Seidel, Julia Bauer, Carsten Geiß, Susanne Gebhard
Topic
Cell Biology, Microbiology
Extract
"For imaging, a Leica DMi8 inverted microscope was used, equipped with a Leica HC PL APO 100×/1.40 oil PH3 objective, CoolLED pE300W light source and a Leica K8 Scientific CMOS camera. Fluorescence of mNeonGreen and msfGFP was excited using the CoolLED pE-300w light source in combination with a GFP filter cube (excitation 470/40 nm, dichroic 495 nm and emission 525/50 nm).
Fluorescence of NR was excited using the CoolLED pE-300w light source together with a TXR filter cube (excitation 560/40 nm, dichroic 585 nm and emission 630/75 nm) at 60% illumination intensity and 50-ms exposure time."
Product Type
Journal
Microbiology
Year of Publication
2026

