Authors

Michael Seidel, Julia Bauer, Carsten Geiß, Susanne Gebhard

Topic

Cell Biology, Microbiology

Extract

"For imaging, a Leica DMi8 inverted microscope was used, equipped with a Leica HC PL APO 100×/1.40 oil PH3 objective, CoolLED pE300W light source and a Leica K8 Scientific CMOS camera. Fluorescence of mNeonGreen and msfGFP was excited using the CoolLED pE-300w light source in combination with a GFP filter cube (excitation 470/40 nm, dichroic 495 nm and emission 525/50 nm).

Fluorescence of NR was excited using the CoolLED pE-300w light source together with a TXR filter cube (excitation 560/40 nm, dichroic 585 nm and emission 630/75 nm) at 60% illumination intensity and 50-ms exposure time."

Product Type

pE-300white

Journal

Microbiology

Year of Publication

2026

Read the full research paper here