Rawan AlSubaie,1 Ryan WS Wee,1 Anne Ritoux,1 Karyna Mishchanchuk,1 Jessica Passlack,1 Daniel Regester,1 and Andrew F MacAskill1
"1 Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom,
Andrew F MacAskill: [email protected]"
"Projections from the basal amygdala (BA) to the ventral hippocampus (vH) are proposed to provide information about the rewarding or threatening nature of learned associations to support appropriate goal-directed and anxiety-like behaviour. Such behaviour occurs via the differential activity of multiple, parallel populations of pyramidal neurons in vH that project to distinct downstream targets, but the nature of BA input and how it connects with these populations is unclear. Using channelrhodopsin-2-assisted circuit mapping in mice, we show that BA input to vH consists of both excitatory and inhibitory projections. Excitatory input specifically targets BA- and nucleus accumbens-projecting vH neurons and avoids prefrontal cortex-projecting vH neurons, while inhibitory input preferentially targets BA-projecting neurons. Through this specific connectivity, BA inhibitory projections gate place-value associations by controlling the activity of nucleus accumbens-projecting vH neurons. Our results define a parallel excitatory and inhibitory projection from BA to vH that can support goal-directed behaviour.
Research organism: Mouse
DOI: https://dx.doi.org/ 10.7554/eLife.74758
Wide-field illumination was achieved via a ×40 objective with brief pulses of blue light from an LED centred at 473 nm (CoolLED pE-4000/Thorlabs M470L4-C1, with appropriate excitation-emission filters)
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The pE-4000 Universal Illumination System offers 16 selectable wavelengths from 365 - 770 nm, making it a highly flexible illuminator covering a wide variety of fluorophores
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