Authors

Stanicki, Dimitri Boutry, Sébastien Laurent, Sophie Wacheul, Ludivine Nicolas, Emilien Crombez, Deborah Vander Elst, Luce Lafontaine, Denis L. J. Muller, Robert N.

Affiliations

Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, B-7000 Mons, Belgium. Centre for Microscopy and Molecular Imaging, B-6041 Gosselies, Belgium cRNA Molecular Biology, Brussels, Belgium.

Application Area

Chemistry

Immunofluorescence

Abstract

This study reports the synthesis of stabilized ultra-small iron oxide nanoparticles (USPIO) as bimodal probes for magnetic resonance and optical imaging. These nanosystems are based on small iron oxide cores surrounded by a thin polysiloxane shell exhibiting carboxylic acid functions. Thanks to these functions, hybrid particles were obtained by conjugating a fluorophore to the super paramagnetic contrastophore. Such modification allowed us to directly follow these USPIO in cellulo, which provided interesting information about their internalization pathway and cellular distribution upon mitosis. Finally, the efficiency of these systems as probes for bimodal imaging was emphasized by the observation of their in vivo behaviour in mice using magnetic resonance and optical imaging.

Extract

… “Cool-LED illuminator and a Roper HQ2 camera. (i) DIC (

Product Associated Features

pE-100: A range of compact, simple-to-use, single wavelength illumination systems for screening fluorescence.

Diascopic Technique

DIC

Live Cell Issues

Cell Viability

Product Type

pE-100

Journal

Journal of Material Chemistry

Year of Publication

2014

Country of Publication

Belgium