Bronchial smooth muscle extracellular vesicles interfere with bronchial epithelium metabolism and function in asthma

Authors

Elisa Celle, Amine Chahin, Fabien Beaufils, Guillaume Cardouat, Edmée Eyraud, Clément Bouchet, Marilyne Campagnac, Olga Ousova, Hugues Begueret, Matthieu Thumerel, Rémi Dubois, Jean-William Dupuy, Thierry Leste-Lasserre, Sabrina Lacomme, Nina Lager-Lachaud, Floriant Bellvert, Roger Marthan, Pierre-Olivier Girodet, Patrick Berger, Thomas Trian, Pauline Esteves

Topic

Cell Biology

Extract

"Ciliary beating frequency of BE were measured using videomicroscopy Leica DMi8 (Leica Microsystems) coupled to a high-speed camera sCMOS Flash 4.0 camera (Hamamatsu) available at The Bordeaux Imaging Center. The illumination system used was Cool LED PE-4000 (CoolLED) associated to a HCX PL Fluotar L 40X dry 0.6 NA PH2 objective. BE were placed in a 37°C chamber using an incubator box equipped with a gas heating system (Pecon GmbH). The acquisitions were carried out during 2 seconds at a frequency of 1000 images/sec so a total of 2001 images, on a defined zone (100x100 pixels) using MetaMorph software (Molecular Devices). Using MATHLAB program, the images were analyzed after application of the Fourier transform to determine the most represented beat frequency."