Bioluminescent Optogenetics: A Novel Experimental Therapy to Promote Axon Regeneration after Peripheral Nerve Injury

Authors

English et al.

Topic

Neuroscience, Optogenetics

Extract

"Patch-clamp recordings were conducted under an upright wide-field fluorescence microscope (Scientifica HyperScope) equipped with an LED (CoolLED pE-300ultra, CoolLED, Andover, MA, USA), a multi-bandpass filter cube (Chroma 89402, Chroma Technology, Bellows Falls, VT, USA), water immersion objective (Nikon CFI75 LWD 16X W 0.8NA), a scientific CMOS camera (OptiMOS, QImaging, Surrey, BC, Canada), a micromanipulator (Scientifica PatchStar, Scientifica, Clarksburg, NJ, USA), a patch-clamp amplifier (Axon MultiClamp 700B, Molecular Devices, San Jose, CA, USA), and a digitizer (Axon Digidata 1550B, Molecular Devices) at room temperature.
"

Product Type

pE-300^ultra

Journal

International Journal of Molecular Sciences

Year of Publication

2021

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