Authors

Arthur W. English,1,2,* Ken Berglund,3 Dario Carrasco,1 Katharina Goebel,1 Robert E. Gross,3 Robin Isaacson,1 Olivia C. Mistretta,1 and Carly Wynans1

Topic

Neuroscience, Optogenetics

Extract

"Patch-clamp recordings were conducted under an upright wide-field fluorescence microscope (Scientifica HyperScope) equipped with an LED (CoolLED pE-300ultra, CoolLED, Andover, MA, USA), a multi-bandpass filter cube (Chroma 89402, Chroma Technology, Bellows Falls, VT, USA), water immersion objective (Nikon CFI75 LWD 16X W 0.8NA), a scientific CMOS camera (OptiMOS, QImaging, Surrey, BC, Canada), a micromanipulator (Scientifica PatchStar, Scientifica, Clarksburg, NJ, USA), a patch-clamp amplifier (Axon MultiClamp 700B, Molecular Devices, San Jose, CA, USA), and a digitizer (Axon Digidata 1550B, Molecular Devices) at room temperature.
"

Product Type

pE-300ultra

Journal

International Journal of Molecular Sciences

Year of Publication

2021