Authors

Suárez, R., Fenlon, L. R., Marek, R., Avitan, L., Sah, P., Goodhill, G. J., & Richards, L. J.

Affiliations

Queensland Brain Institute School of Biomedical Sciences School of Mathematics and Physics
The University of Queensland, Brisbane, QLD 4072, Australia.

Application Area

Optogenetics

Electrophysiological Techniques

Abstract

Bilateral integration of sensory and associative brain processing is achieved by precise connections between homologous regions in the two hemispheres via the corpus callosum. These connections form postnatally, and unilateral deprivation of sensory or spontaneous cortical activity during a critical period severely disrupts callosal wiring. However, little is known about how this early activity affects precise circuit formation. Here, using in utero electroporation of reporter genes, optogenetic constructs, and direct disruption of activity in callosal neurons combined with whisker ablations, we show that balanced interhemispheric activity, and not simply intact cortical activity in either hemisphere, is required for functional callosal targeting. Moreover, bilateral ablation of whiskers in symmetric or asymmetric configurations shows that spatially symmetric interhemispheric activity is required for appropriate callosal targeting. Our findings reveal a principle governing axon targeting, where spatially balanced activity between regions is required to establish their appropriate connectivity.

Extract

… “border contralateral to the electroporated hemisphere using an LED system (pE-2, CoolLed).”…

Product Associated Features

pE-2: A repeatable, controllable modular system with 20 different LED peaks. Instant on/off and intensity (0-100%) control.

Product Type

-

Journal

Neuron

Year of Publication

2014

Country of Publication

Australia