Authors

Giuseppe D’Agostino1,2*, David J Lyons1, Claudia Cristiano1, Luke K Burke2,
Joseph C Madara3, John N Campbell3, Ana Paula Garcia2, Benjamin B Land4,
Bradford B Lowell3, Ralph J Dileone4, Lora K Heisler1,2*

Topic

Neuroscience

Extract

"Photostimulation of channelrhodopsin2 was achieved by 470 nm blue light delivered via the microscope objective. Light was generated by a pE-4000 LED illumination system (CoolLED, Andover, UK) driven via clampex 10.4 software (Molecular Devices, Sunnyvale, CA). For CCK-8 application on MC4R PVH neurons, brain coronal slices from 6–8 week-old Mc4r-GFP or Mc4r-t2a-Cre::tdTomato mice were prepared. Extracted brains were immediately submerged in ice-cold, carbogen-saturated (95% O2, 5% CO2) high-sucrose solution (238 mM sucrose, 26 mM NaHCO3, 2.5 mM KCl, 1.0 mM NaH2PO4, 5.0 mM MgCl2, 10.0 mM CaCl2, 11 mM glucose)"

Product Type

pE-4000

Journal

eLife

Year of Publication

2016