As areas become increasingly urbanised, it is crucial to understand the effects of particulate matter in the air we breathe. Professor Yang from the Department of Physiology, Gachon University College of Medicine in Korea, explains how he uses the CoolLED pE-340fura to examine the correlation between particulate matter and cellular damage.
Aim: Particulate matter (PM) air pollution is notably related to morbidity worldwide. Our study examines the correlation between PM and cellular damage, investigating PM-modulated intracellular Ca2+ signalling and searching for therapeutic candidates which can antagonize the effects of PM.
Findings: Human keratinocytes and airway epithelia cell lines challenged with diesel PM turn on the protease-activated receptor-2 (PAR-2) signalling pathway, leading to oxidative stress via production of reactive oxygen species and secretion of pro-inflammatory cytokines. Furthermore, we found out that some natural extracts can inhibit DPM triggered Ca2+ influx and PAR-2 activation, protecting skin and airway cells from air pollution.
Conclusion: The novel concept of Ca2+ influx and following signal transductions in human keratinocytes and respiratory epithelia will provide a working platform to explore natural products, targeting human skin and airway disease.
We would like to thank one of our Resellers in Korea, Magic Tree, for supporting this researcher.