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Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE

In the early days of LED adoption for fluorescence microscopy, many people saw LED’s as simply a light source. Therefore, the available LED wavelengths were geared towards traditional fluorophores. However, recent systems have offered greater flexibility that can open up other fluorophore options. One fluorophore example is quantum dots, which have the advantage of being bright and having greater stability compared to other fluorophores.

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