Overview

Excitation filters are always recommended when using pE-Series LED illumination systems for fluorescence microscopy. Successful fluorescence microscopy depends on matching filters with the source of illumination and the stain to be excited. These filters are commonly included within filter cubes in the microscope.

optical_imgBenefits of excitation filters

CoolLED specialize in LED illumination systems for microscopy. We know that to get the best results from your microscope you need to match the right filters to your illumination system and samples.

Although LEDs produce a narrow spectral peak of light, there are often regions of shorter and longer wavelength illumination which may lead to increased background in the image or interfere with the excitation of another stain if no excitation filter is used.

When selecting an LED illumination system which is based on a single, or few wavelength peaks, it is essential that care is taken to match these with the excitation filter in the microscope. Alternatively, a new filter can be fitted which is matched to the illumination system and the stain.

Broad spectrum illumination systems are less sensitive to this optimisation requirement but it is still beneficial to check the filters. With modular LED systems, CoolLED’s wide range of LED wavelengths (LAMs) means that an LED wavelength can be specified which matches with the existing filters in the microscope.

Our products will work with most filter sets out there, but for optimum results we have made some suggestions below. There is never a definite right answer as depending on the demands of your setup you may want more brightness, more contrast or to satisfy some other requirement.

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Above are suggested filter sets from Chroma and Semrock for popular fluorophores. In most cases performance is similar, but those looking for particular brightness or contrast are urged to examine the manufacturer’s specifications further or ask CoolLED for help.